复方中药结合预运动训练对运动热应激大鼠脑组织氧化损伤的保护作用  

Chinese Herbal Medicine Combined with Pre-training Protect Rat Brain Neurons against Oxidative Damage during Exercise in the Heat

作　　者：王大磊[1] 胡琳茹 王童[1] 郭成吉[3] 

机构地区：[1]国防科学技术大学体育与基层工作系,湖南长沙410003 [2]北京科技大学土木与环境工程学院,北京100083 [3]曲阜师范大学体育科学学院,山东曲阜273165

出　　处：《北京体育大学学报》2014年第2期66-70,共5页Journal of Beijing Sport University

摘　　要：目的:对不同干预方法改善运动热应激大鼠脑组织氧化损伤及运动能力的作用进行观察。方法:雄性Wistar大鼠,被随机分为安静对照组(A组)、运动对照组(B组)、安静给药组(C组)和运动给药组(D组),每组12只,其中8只用于高温环境下的定量运动实验,4只用于力竭运动实验。B、D组进行5周预运动训练,C、D组每日灌服复方中药(共5周),干预结束后所有大鼠分别于高温环境下进行1 h定量运动或力竭运动。定量运动大鼠运动结束后即刻断头取脑对皮层羟自由基抑制能力、丙二醛(MDA)、超氧化物歧化酶(SOD)、黄嘌呤氧化酶(XOD)、谷胱甘肽过氧化物酶(GPX)活性及热应激蛋白70(HSP70)表达情况进行观察。记录力竭运动过程中大鼠肛温变化及运动至力竭时间。结果:3种干预方法均能够降低高温环境下运动1 h大鼠脑组织MDA、XOD水平(P<0.05),提高其羟自由基抑制能力、SOD、GSH-PX及HSP70表达(P<0.05),延缓核心温度升高速率并延长运动至力竭时间(P<0.05)。从效果来看,两者结合干预效果优于单纯预运动训练,后者又优于单独复方中药。结论:3种干预方法均能起到降低高温环境下脑组织氧化损伤及提高运动能力的作用,且联合干预方法优于单独干预方法。脑组织HSP70表达增加可能是3种干预方法发挥作用的重要、共同途径之一。Purpose: To observe the effect of three different interventions on improving the oxidative stress level in rat brain and the exercise capacity in heat environment. Methods: Wistar male rats were randomly divided into rest control group( A group),exercise control group( B group),rest herbal medicine group( C group) and exercise combined herbal medicine group( D group). Each group consisted of 12 rats,8 of them suffered the fixed volume of exercise,and 4 of them suffered the exhaustive exercise. Rats in B,D group participated in a 5 weeks pre-training protocol. During the 5 weeks,rats in the C and D group were drenched with Chinese herbal medicine. After 5 weeks,all rats respectively participated in fixed volume exercise or exhaustive exercise in heat environment. After the fixed volume exercise,rats were decapitated immediately and the concentrations / activity of anti-hydroxyl radical,MDA,SOD,XOD,GPX and HSP70 expression in rat motor cortex were observed. During the process of doing exhaustive exercise,the rectal temperature and exercise time till exhaustion were recorded. Results: After pretraining,Chinese herbal medicine and the combined intervention,the MDA and XOD concentration / activity significantly decreased in rat brain after the fixed volume exercise in heat( P < 0. 05),the anti-hydroxyl radical, SOD,GPX and HSP70 expression significantly increased( P < 0. 05),the body temperature rising rate and theexercise time till exhaustion significantly decreased( P < 0. 05). The effect of the combined intervention was better than the single exercise intervention,and the singe exercise intervention was better than the single Chinese herbal medicine intervention. Conclusion: The pre-training,Chinese herbal medicine and the combined interventions were all effective on improving the oxidative stress level in brain and prolong the exercise capacity in heat environment. The combined intervention was better than the individual ones. The increased expression of HSP70 might be important and common approach for the three i

关 键 词：复方中药 预运动训练 运动热应激 脑组织 氧化损伤 

分 类 号：R87[医药卫生—运动医学]