对虾白斑杆状病毒(WSBV)定量PCR检测技术研究  

Research of Quantitative PCR Detection Technology of White Spot Bacilliform Virus (WSBV)

作　　者：徐丽美[1] 王玮[1] 杨丰[1] 

机构地区：[1]国家海洋局第三海洋研究所,厦门361005

出　　处：《高技术通讯》2001年第1期14-16,共3页Chinese High Technology Letters

基　　金：86 3计划!(819 0 2 0 4);福建省自然科学基金!(C9810 0 39)资助项目

摘　　要：报道了利用DNA重组技术在一段对虾白斑杆状病毒 (WhiteSpotBacilliformVirus ,,WSBV)的基因组DNA片段中缺失 74bp ,重组得到的内标质粒作为竞争性内标模板 ,并与阳标模板或病虾DNA模板共同扩增 ,建立了定量PCR检测方法。研究表明 ,定量PCR方法检测灵敏度达 10病毒分子DNA/ μl,样品DNA模板制备回收率约 50 % 。A DNA fragment of White Spot Bacilliform Virus which is lack of 74 bp was preperred and used as a competitive internal standard DNA which is amplified with the positive template or infected shrimp DNA template to study its recovery and determine the content of WSBV in the infected shrimp. The results showed that the sensitivity of the quantitave PCR reach to the Virus DNA of 10 molecular/μl. The recovery was estimated at about 50%.

关 键 词：定量PCR 对虾白斑杆状病毒 WSBV 内标模板 检测 

分 类 号：S945[农业科学—水产养殖]