表达Harpin蛋白质Hpa1功能片段的转基因小麦对赤霉病的抗性(英文)  

Transgenic expression of an active fragment of the harpin protein Hpal in wheat reduces Fusarium head blight

作　　者：李小杰[1] 王小冰[1] 王德福[1] 田珊[1] 董汉松[1] 张春玲[1] 

机构地区：[1]南京农业大学,作物病虫监测与治理教育部重点实验室,南京210095

出　　处：《植物保护学报》2014年第1期25-34,共10页Journal of Plant Protection

基　　金：国家转基因生物育种重大专项(2013ZX08002001-007)

摘　　要：来自水稻黄单胞菌的harpin蛋白质Hpa1有促进植物生长、诱导植物抗病性的功能，Hpa1序列10-42氨基酸片段（Hpa110-42）的活性比全长分子高1.3-7.5倍。为研究Hpa110-42在转基因小麦体内表达以后对赤霉病的影响和评价转基因小麦抗病水平与应用潜力，对6个小麦转基因系进行了测定。结果显示，Hpa110-42在转基因系T3-T5代呈现稳定表达，用禾谷镰刀菌一个分离物进行接种以后，转基因系发生赤霉病的程度较非转基因小麦显著降低，且转基因系T3-T5代赤霉病降低的趋势一致。另外，转基因系小麦病害减轻的程度，与在非转基因小麦上使用杀菌剂的效果相当，表明Hpa110-42转基因表达对小麦赤霉病有防治作用。The harpin protein Hpa1 produced by a bacterial pathogen of rice promotes plant growth and induces disease resistance, and in both effects the 10-42-residue fragment （Hpa110-42） isolated from the Hpa1 sequence is 1.3-7.5-fold more effective than the full length. To evaluate possible role of Hpa110-42 in wheat resistance to the Fusarium head blight disease, the Hpa110-42 gene was transferred into the crop genome, followed by resistance assessments under greenhouse conditions. The independent experiments showed that transgenic expression of Hpa110-42 in wheat reduced the disease severity. Six Hpa110-42-expressing wheat lines were tested and the expression of Hpa110-42 was found to be consistent in T3-T5 progenies. Following inoculation with an indigenous F.graminearum isolate, transgenic lines had significantly lower levels of Fusarium head blight compared to non-transformed plants. Reduction of disease severity was consistent in T3-T5 progenies of Hpa110-42-expressing lines and was equivalent to that in non-transformed plants treated with a fungicide. Therefore, transgenic Hpa110-42 expression might provide an effective measure for management of Fusarium head blight in the agriculturally significant crop.

关 键 词：harpin蛋白质 Hpa110-42 转基因小麦 赤霉病 病害控制 

分 类 号：S435.121.45[农业科学—农业昆虫与害虫防治]