二斑叶螨对阿维菌素的抗药性及抗性基因的PASA检测技术  

Abamectin resistance of Tetranychus urticae and detection for resistance-related gene by PASA technique

作　　者：唐小凤[1] 王少丽[1] 张友军[1] 吴青君[1] 谢文[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《植物保护学报》2014年第1期67-73,共7页Journal of Plant Protection

基　　金：国家"863"项目(2012AA101502);公益性行业(农业)科研专项(201103020);农业部园艺作物生物学与种质创制综合性重点实验室

摘　　要：为了明确二斑叶螨Tetranychus urticae Koch对阿维菌素的抗性水平,采用玻片浸渍法测定了北京4个地区二斑叶螨雌成螨对阿维菌素的抗药性,建立了特异性等位基因PCR(PASA)方法,并检测了二斑叶螨谷氨酸门控氯离子通道(GluCl)基因片段上G323D的突变频率。结果显示,北京昌平、海淀、密云和怀柔4个田间二斑叶螨种群对阿维菌素均达极高抗性水平,其中昌平种群抗性最高,LC50值为448.93 mg/L,抗性倍数为4988.11倍。室内敏感种群未见抗性个体,昌平和密云种群G323D等位基因突变频率为100.00%,怀柔和海淀种群分别为86.25%和90.00%。北京4个地区二斑叶螨种群对阿维菌素达极高抗性水平,抗性基因的突变频率也很高,表明阿维菌素不适宜用来防治这些地区的二斑叶螨种群。To estimate the abamectin resistance level of Tetranychus urticae Koch, bioassay was conducted against abamectin on the adult females of T.urticae from 4 districts in Beijing area using slide-dip method. The PCR amplification of specific alleles （PASA） was developed to detect the point mutation frequency of G323D in glutamate-gated chloride channel （GluCl） gene. The results showed that the field populations collected from Changping, Haidian, Miyun and Huairou in Beijing exhibited extremely high resistance level. Especially the Changping population was proved to be the highest resistance to abamectin with the LC50 value of 448.93 mg/L, showing 4 988.11-fold. Resistance genotype was not detected in the susceptible strain. However, the mutation frequency in Changping and Miyun populations of G323D allele was 100.00%, and those in Huairou and Haidian populations were 86.25% and 90.00%. Abamectin is not suitable for controlling T.urticae populations in the 4 tested areas in Beijing due to the extremely high resistance and high frequency of resistant genotype.

关 键 词：二斑叶螨 阿维菌素 抗药性 谷氨酸门控氯离子通道 G323D点突变 

分 类 号：S433[农业科学—农业昆虫与害虫防治]