抗啶酰菌胺蔬菜灰霉病菌突变体的诱导及其生物学性状  

Induction and characteristics of Botrytis cinerea resistant mutants to boscalid

作　　者：任璐[1] 余玲[1] 韩巨才[1] 刘慧平[1] 

机构地区：[1]山西农业大学农学院,太谷030801

出　　处：《植物保护学报》2014年第1期87-92,共6页Journal of Plant Protection

基　　金：山西省自然科学基金(2013011032-2);山西省留学基金项目(2011-52);山西省科技攻关项目(20110311013-1)

摘　　要：为评价蔬菜灰霉病菌对啶酰菌胺的抗性风险,采用紫外线照射和药剂驯化相结合的诱导方法,对蔬菜灰霉病菌D9、L17、J9亲本敏感菌株进行了室内啶酰菌胺抗性诱导,并采用菌落直径法比较了抗、感菌株在生物学性状方面的差异。结果显示,共获得不同抗性水平的突变体23株,其中突变体D9-4的抗性倍数为253.76倍,达高抗水平。突变体菌丝生长速率较敏感菌株有所下降,抗、感菌株的适宜生长温度均为25℃,敏感菌株最适pH为5,突变体最适pH为7,突变体适应酸碱度和温度变化的能力较敏感菌株差;低抗突变体产孢量和孢子萌发率与敏感菌株无显著差异,但高抗突变体产孢量和孢子萌发率较敏感菌株显著降低;在无药条件下,敏感菌株致病力强于抗性突变体,而在药剂胁迫下,突变体表现出一定的耐药性,致病力强于敏感菌株;6株突变体无药继代培养15代后,突变体抗性稳定遗传。此外,蔬菜灰霉病菌对啶酰菌胺与其它5种杀菌剂之间没有交互抗性。研究表明,蔬菜灰霉病菌抗啶酰菌胺突变体适合度较低,但在药剂选择压力下容易形成啶酰菌胺抗性群体,在生产中需引起注意。To determine the resistance risk of Botrytis cinerea to boscalid, three susceptible strains, D9, L17 and J9, were collected and induced by UV irradiation and fungicide adaption in the laboratory. As a result, 23 boscalid-resistant mutants were obtained, and the resistance of mutant D9-4 to boscalid reached 253.76 folds compared with the susceptible baseline, up to high level. The characteristics of resistant mutants and sensitive isolates were studied by measuring the colony diameters. The mycelium growth of resistant mutants declined compared with sensitive strains. For the growth of sensitive isolates and resistant mutants, the optimal temperature was 25 ℃, the optimal pH value of sensitive isolates was 5 and that of the mutants was 7. Meanwhile, it was more difficult for the resistant mutants to become adapted to changes in pH and temperature. Pathogenicity of the mutants was weaker than that of the sensitive ones without boscalid, but stronger with bosclaid, because mutants could tolerate a certain dose of fungicide. The resistance level of the 6 mutants was stable after successive incubations for 15 generations on the fungicide free of PDA. Meanwhile, there was no cross-resistance to the other 5 fungicides. The fitness of resistant mutants was lower than that of the sensitive strains, but B.cinerea treated with boscalid was prone to producing resistant populations, and attention should be paid to the resistance risk in production.

关 键 词：灰霉病菌 啶酰菌胺 抗药性风险 生物学性状 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]