花生高效再生体系的建立初报  

Establishment of Efficient Regeneration System of Peanut

作　　者：尤淑丽 

机构地区：[1]辽宁省风沙地改良利用研究所,辽宁阜新123000

出　　处：《黑龙江农业科学》2014年第3期13-15,共3页Heilongjiang Agricultural Sciences

基　　金：辽宁省科技厅攻关资助项目(2011201021)

摘　　要：为了建立花生高效再生体系，选用外引及自选共8个花生品种（系）胚小叶作外植体，研究愈伤组织诱导率和不定芽分化率。结果表明：品种间愈伤组织诱导率和不定芽分化率均差异显著。品种冀花4号愈伤组织诱导率最高，为98％；太空1号、唐油4号、豫花9626、花育20较高，泉花646、阜花13次之；品系452—2最低，为43％。不定芽分化率为5％～73％，其中冀花4号最高，为73％；太空1号68％较高；豫花9626次之，为35％；花育20、泉花646和452—2较低，分别为13％、11％和10％；阜花13、唐油4号最低，均为5％。In order to establish the efficient regeneration system of peanut, taking embryonic leaflets of eight peanut varieties as explants,the effects of genotypes on callus formation rate and buds differentiation rate were analyzed. The results showed that there were significant effects of peanut genotype on callus formation ra;e and buds differentiation rate. Callus formation rate of j ihua 4 was the hightest, 98 %;Taikong 1, Tangyou 4, Yuhua 9626 and Huayu 20 were higher;Quanhua 646 and Fuhua 13 were lower,452-2 was the lowest,achieved 43%. The buds differentiation rate were the range of 50%073 %, the buds differentiation rate of Jihua 4 was the high- test, achieved 73 % - Taikongl achieved higher, 68 % ; Yuhua 9626 was lower, achieved 35 % ; Huayu 20, Quanhua 646,452-2 were lower,achieved 13 % and 11% and 10 ; respectively; Fuhua 13 and Tangyou 4 were the low- est,achieved 5 %.

关 键 词：花生 基因型 愈伤组织诱导 不定芽分化 

分 类 号：S565.2[农业科学—作物学]