长期留置管细菌分布及生物膜形成能力调查  

The distribution and biofilm formation abilities of bacteria isolated from long term indwelling tubes

作　　者：程绘珺 林凡[2] 徐瑶瑶[1] 张日伊 俞刚[1] 许柏芸 周燕[3] 楼永良[1] 杜季梅[1] 

机构地区：[1]温州医科大学检验医学院,浙江温州325035 [2]温州医科大学第一临床学院,浙江温州325035 [3]温州医科大学生物学实验教学中心,浙江温州325035

出　　处：《中国微生态学杂志》2014年第2期163-165,共3页Chinese Journal of Microecology

基　　金：2012年浙江省教育厅项目(Y201223986);2012年国家级大学生创新创业训练计划项目(201210343001)

摘　　要：目的了解长期留置管细菌分布及生物膜形成能力。方法 10%血清肉汤振荡培养留置管中细菌,常规生化和分子生物学方法鉴定分离菌,结晶紫半定量法检测分离菌生物膜形成能力。结果留置管细菌检出率为49.1%(53/108);共分离60株菌,以葡萄球菌分离率最高(70.0%),其次为肠球菌(15.0%)和大肠埃希菌(6.7%);所有分离菌均具有生物膜形成能力,其中38株(63.3%)具有较强的生物膜形成能力。结论长期留置管细菌检出率较高,大部分分离菌具有较强的生物膜形成能力。Objective To reveal the distribution and biofilm forming ability of bacteria isolated from longtime in- dwelling tubes. Methods Bacteria from longtime indwelling tubes were enriched and shaking cultured in broth containing 10% calf serum, then isolated and identified by routine method and PCR. The biofilm formation capa- bility of each isolated bacterium was detected by crystal violet semi-quantitative method. Results The bacterial detection rate of the longtime indwelling tubes was 49.1% （53/108）. Among the 60 strains of bacteria isolated, the most frequently occurred bacteria was Staphylococcus （70%） , followed by Enterococcus （15.0%） and Esche- richia coli （6.7%）. All the strains isolated in this study had biofilm forming ability, 38 （63.3%） of which had strong capabilities of biofilm formation. Conclusion The bacterial detection rate of longtime indwelling tubes is remarkably high and most isolates have strong biofilm forming abilities.

关 键 词：留置管 细菌分布 生物膜 

分 类 号：R378[医药卫生—病原生物学]