转染从灰飞虱提取的Wolbachia对豆叶螨繁殖适合度和寿命的影响  

Effects of transinfection of Wolbachia from Laodelphax striatellus( Hemiptera: Delphacidae) on reproductive fitness and adult longevity of Tetranychus phaselus( Acari: Tetranychidae)

作　　者：赵冬晓[1,2] 张艳凯[1] 陈汉[1] 洪晓月[1] 

机构地区：[1]南京农业大学昆虫学系,南京210095 [2]南通市通州区农业委员会,江苏南通226300

出　　处：《昆虫学报》2014年第1期25-35,共11页Acta Entomologica Sinica

基　　金：国家公益性行业(农业)科技项目(200903051;200803003)

摘　　要：【目的】Wolbachia是广泛存在于节肢动物体内的一类母系遗传的共生菌,能够通过多种机制调节节肢动物的生殖。近年来,为了更进一步地探究Wolbachia与寄主之间的互作机制,许多研究者展开了Wolbachia的人工转染研究。【方法】我们在实验室条件下将灰飞虱Laodelphax striatellus(Fallén)感染的Wolbachia提取纯化后,利用显微注射的方法导入豆叶螨Tetranychus phaselus Ehara体内。研究了注入从灰飞虱提取的Wolbachia和豆叶螨自然感染Wolbachia对豆叶螨繁殖适合度和寿命的影响,并测定了两种Wolbachia的密度随豆叶螨日龄增长的变化情况。【结果】结果显示,外源Wolbachia在豆叶螨体内的拷贝数极低,仅为自然感染豆叶螨体内Wolbachia拷贝数的0.5%左右。与自然感染的Wolbachia不同,外源Wolbachia在豆叶螨种群中不能引起胞质不亲和,但能够显著降低雌螨的产卵量。【结论】本研究表明,来自灰飞虱的Wolbachia具有抑制豆叶螨种群扩张的潜在能力,对豆叶螨生物防治具有一定的应用价值。[ Aim] Wolbachia are a group of maternally inherited intracellular bacteria that infect a wide range of arthropods and cause an array of effects on host reproduction. Recently, researchers have carried out transfer experiments that generated novel infection types to elucidate the interaction between Wolbachia and the hosts. [ Methods] Under laboratory conditions, Wolbachia infected by the small brown planthopper, Laodelphax striatellus （Fallén）, were extracted and purified, and then were transferred into the new host Tetranychus phaselus Ehara using microinjection. The effects of Wolbachia extracted from L. striateUus and the native Wolbachia on reproductive fitness and adult longevity of T. phaselus were studied, and the densities of Wolbachia during host aging were measured. [ Results] Our results showed that the novel Wolbachia was present at a rather low density in T. phaselus, being only 0.5% of the densities of the native Wolbachia. Unlike native Wolbachia, novel Wolbachia could not induce cytoplasmic incompatibility in the T. phaselus population, but they could decrease the fecundity of T. phaselus significantly. [ Conclusion ] This study indicates that Wolbachia from L. striatellus have potential ability to inhibit population expansion of T. phaselus and will be valuable for biocontrol of T. phaselus.

关 键 词：灰飞虱 豆叶螨 共生菌 WOLBACHIA 显微注射 人工转染 繁殖适合度 寿命 

分 类 号：Q965.8[生物学—昆虫学]