STAT3在肝细胞增殖过程中对C/ebpβ表达的影响  

Effects of STAT3 on C/ebpβ expression in the process of hepatocyte proliferation

作　　者：匡怡 刘孟刚[1] 魏东[1] 陈红旭[1] 李光耀[1] 陈平[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所肝胆外科,重庆400042

出　　处：《免疫学杂志》2014年第3期202-205,210,共5页Immunological Journal

基　　金：国家自然科学基金面上项目(81270523)

摘　　要：目的探讨STAT3在IL-6刺激肝细胞增殖过程中对C/ebpβ表达变化的影响。方法用含15 ng/ml的IL-6培养基培养大鼠正常肝细胞BRL-3A,运用CCK试剂盒检测该细胞在IL-6刺激后不同时间里的增殖情况,Western blot技术检测STAT3、P-STAT3、C/ebpβ的蛋白表达变化情况;利用siRNA瞬时转染技术抑制STAT3在BRL-3A细胞中的表达并观察IL-6刺激肝细胞增殖过程中C/ebpβ的表达变化。结果 IL-6能有效的刺激肝细胞增殖,在增殖早期STAT3、P-STAT3、C/ebpβ表达均明显上调;在肝细胞增殖过程中沉默STAT3后C/ebpβ表达下调。结论在IL-6肝细胞增殖过程中STAT3对C/ebpβ起正向调控作用。This study performed to investigate the effect of STAT3 on C/ebpβ expression in hepatocyte proliferation process stimulated by IL-6. We used CCK kits to detect the proliferation of normal rat liver cell BRL- 3A, which were cultured with medium containing 15 ng/ml IL-6 for different times; then Western blot was employed to evaluate the changes of the expression of STAT3, P-STAT3, and C/ebpβ. Subsequently, the expression of STAT3 in BAL-3A cell was suppressed by siRNA transient transfection technique and the change of C/ebpβ expression was observed in the process of hepatocyte proliferation stimulated by IL-6. Data suggested that IL-6 could effectively stimulate the hepatocyte proliferation, and the protein expression of STAT3, P-STAT3, and C/ebpβ were significantly increased in the early stage of hepatocyte proliferation; the expression of C/ebpβ would down-regulated in the process of hepatocyte proliferation when STAT3 silenced. The result indicated that STAT3 has a positive regulatory effect on C/ebpβ in the process of hepatocyte proliferation stimulated by IL-6.

关 键 词：肝再生 STAT3 P-STAT3 C ebpβ IL-6 

分 类 号：R657.3[医药卫生—外科学] R394.2[医药卫生—临床医学]