硫辛酸抑制脂多糖诱导的星形胶质细胞细胞因子及趋化因子的表达  

Effects of lipoic acid on cytokines and chemokines in astrocytes stimulated with lipopolysaccharide

作　　者：孟健[1] 郭敏芳[1] 尉杰忠[1] 李艳花[1] 刘春云[1] 侯绍蔚[1] 丰玲[1] 杨琬芳[2] 李俊莲[2] 冯前进[2] 肖保国[1,3] 马存根[1,2] 

机构地区：[1]山西大同大学脑科学研究所,山西大同037009 [2]国家卫生部临床重点专科,山西中医学院第三中医院脑病科,山西太原030024 [3]复旦大学华山医院神经病学研究所

出　　处：《细胞与分子免疫学杂志》2014年第4期346-350,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：2013年度国家科技部国际科技合作专项项目(2013DFA30700);山西省回国留学人员科研资助项目(2008-86);山西大同大学博士科研启动经费(2008-b-21);山西省基础研究计划青年项目(2012021034-2);山西中医学院"2011"培育计划资助项目(2011PY-1);山西大同大学校级科研项目(2007k10)

摘　　要：目的探讨硫辛酸(LA)对脂多糖(LPS)激活的星形胶质细胞分泌TNF-α、IL-1β、IL-6和IL-10及相关趋化因子的影响。方法分离并鉴定新生C57BL/6小鼠大脑皮质星形胶质细胞,1μg/mL LPS刺激第2代星形胶质细胞,100μg/mL LA进行干预,Griess法检测NO的分泌,ELISA检测TNF-α、IL-1β、IL-6和IL-10炎性因子的含量,反转录PCR检测炎症趋化因子CC亚族趋化因子配体20(CCL20),单核细胞趋化蛋白1(MCP-1)和巨噬细胞炎性蛋白1α(MIP-1α)mRNA的表达。结果与正常组比较,LPS刺激星形胶质细胞后,NO、TNF-α、IL-1β、IL-6分泌显著升高,IL-10分泌下调(P<0.05);LA能抑制LPS诱导的NO、TNF-α、IL-1β、IL-6的分泌,增加IL-10的分泌,与LPS组相比差异有统计学意义(P<0.05)。LA能显著下调LPS所致的CCL20、MIP-1α、MCP-1 mRNA的分泌。结论 LA能抑制LPS激活星形胶质细胞所致的炎性反应,其作用与抑制炎性因子及趋化因子的分泌有关。Objective To investigate the effects of lipoic acid （LA） on the release of TNF-a, IL-I~, IL-6, IL-10 and the expressions of chemokines in astrocytes stimulated with lipopolysaccharide （LPS）. Methods Astrocytes were separated from the cerebral cortex of newly-born CS？BL/6 mice （within 48 h after birth ）. After identification and purification, the second-generation astrocytes were stimulated with LPS （ l pg/mL）, and then treated with LA （ 100 iJg/mL）. The production of nitric oxide （NO） was assayed by Griess assay. The levels of TNF-a, IL-1[3, IL-6 and IL-I0 in supernatants were quantified by ELISA. The expressions of CC chemokine ligand-20 （CC1_20）, monocyte chemoattractive protein 1 （MCP-1） and macrophage inflammatory protein-Ice （MIP-Ia） mRNAs were detected using reverse＇transcription-PCR. Results ~red with PBS control, LPS significantly increased the production of TNF-a, IL-II~ and IL-6, but decreased the level of IL-10 in cultured astrocytes （P 〈 0.05）. LA treatment inhibited LPS-induced NO, TNF-a, IL-115 and IL-6 production, and enhanced IL-IO secretion, and compared with LPS stimulation alone, the differences were statistically significant （P 〈 0.05 ）. In addition, LA treatment also suppressed the expressions of CC1_20, MCP-1 and MIP-1a mRNA in astrocytes stimulated with LPS. Conclusion LA inhibits neuroinflammatory response in LPS-activated astrocytes. The neuroprotecUon of LA is partly due to the inhibition of pro-inflammatory cytokines and chemokines derived from astrocytes.

关 键 词：硫辛酸 脂多糖 细胞因子 

分 类 号：R446.61[医药卫生—诊断学] R392.11[医药卫生—临床医学]