正常成年C57小鼠短声诱发听性脑干反应测试方法与波形分析  

Method and Analysis of Click Induced Auditory BrainstemResponses of Normal Adult Mice

作　　者：李登科[1] 赵立东[1] 郭维维[1] 于宁[1] 孙伟 杨仕明[1] 

机构地区：[1]中国人民解放军总医院耳鼻咽喉头颈外科,北京100853 [2]美国纽约州布法罗大学

出　　处：《听力学及言语疾病杂志》2014年第2期145-147,共3页Journal of Audiology and Speech Pathology

基　　金：国家973计划重大科学研究计划干细胞项目(2012CB967900);国家973计划重大科学问题导向项目(2011CBA01000);国家863计划专题项目(2007AA02Z150);国家自然科学基金重点项目(30730040)资助

摘　　要：目的分析正常C57小鼠短声刺激诱发听性脑干反应（ABR）各波的出现率以及潜伏期、阈值和幅值，为C57小鼠听觉研究提供参考。方法对20只C57小鼠进行短声（click声）诱发的ABR测试？观察高（90dBSPL）、中（40dBSPL）、低（20dBSPL）刺激强度下各波的出现率，以出现率最高的波来判断阈值，同时量取该波的潜伏期和幅值。结果①在高、中与低刺激声强下波Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ的引出率分别为100％、100％、100％、75％、65％，75％、100％、100％、45o．4、40％，30％、90％、40％、5％、0％；波Ⅱ引出率最高，最稳定，最适合用来判断反应阈，其平均阈值为20dBSPL；②刺激声强度从90dBSPL逐渐降低到20dBSPL时，波II潜伏期在2．71～3．08ms之间，幅值在6．20～1．63μV之间。结论C57小鼠短声诱发ABR波Ⅱ出现率最高，最稳定，应以刚刚引出波Ⅱ的刺激声强度作为其反应阈。Objective To obtain the auditory brainstem response （ABR） wave of normal adult mice, and to analyze the thresholds, latency and amplitudes to provide reference for future mice auditory research. Methods To determine the thresholds according to the high occurrence rate wave of click ABR at high（90 dB SPL）, moderate （40 dB SPL）and low（20 dB SPL）sound intensities, thresholds, latencies and amplitudes decreased as the wave with high occurrence rate. Results The occurrence rates of wave Ⅰ、Ⅱ、Ⅲ、Ⅳ、and Ⅴ were 100%,100%,100%,75% 6500 at high levels; 75% ,100% ,100% ,45% ,40% at moderate levels, and 30% ,90% ,40% ,5% ,0% at low levels, respec- tively. The results showed wave morphology was suitable to judging thresholds., the average threshold was 20＋ 5.62 dB SPL,latency and amplitude ranged from 2.71±0. 17-3. 08±021 ms and 6.20±2. 24-1.63±0. 85 μV, respectively, when sound intensities changed from 90 to 20 dB SPL . Conclusion Wave II is regarded as the best wave to determine threshold latency and amplitude. It will help future researches about mice auditory under pathophysiology conditions.

关 键 词：小鼠 听性脑干反应 潜伏期 反应阈 幅值 

分 类 号：R764.04[医药卫生—耳鼻咽喉科]