大鼠糖尿病模型的建立及其视网膜功能早期改变的研究  

Establishing diabetic rat model and early changes of the visual function

作　　者：丁慰祖[1] 姚慧萍[1] 刘嫣[1] 钱钧[1] 许宇东[1] 叶芳[1] 

机构地区：[1]江苏大学附属上海第八人民医院眼科,上海200235

出　　处：《临床眼科杂志》2014年第1期80-82,共3页Journal of Clinical Ophthalmology

摘　　要：目的建立大鼠糖尿病模型并观察实验性糖尿病大鼠视网膜功能早期改变。方法采用腹腔注射链脲佐菌素(STZ)诱导方法建立大鼠糖尿病模型,对大鼠眼部情况、血糖及体重等一般情况进行观察。对实验组和对照组大鼠进行视网膜电图检测,并对各指标进行统计学分析。结果成功建立大鼠糖尿病模型,一次成模率高,大鼠死亡率较低。建模6周起裂隙灯显微镜检查可观察到部分大鼠晶状体皮质混浊,眼底检查未发现改变。建模后1周闪光视网膜电图(FERG)的a、b波潜伏期即较对照组显著延长(P<0.01),但振幅未见异常。而6周时糖尿病组FERG的a、b波除潜伏期持续延长外,其振幅也显著下降(P<0.01)。结论链脲佐菌素诱导的大鼠糖尿病模型建模简单、方便,成功率高,是较为理想、稳定的研究早期糖尿病视网膜病变的动物模型之一。大鼠糖尿病早期在眼底出现视网膜可见病变之前即已存在功能改变。Objective To establish diabetic rat model and observe early changes of visual function. Methods Diabetic rat model was established by intraperltone~ injection of STZ. General condition and eleetroretinograph of the dia- betic group and control group were observed and analyzed. Results The rat diabetic model was well established with high achievement ratio and low mortality. Cortex of lens opacities was observed from 6 weeks and on. There were no visible changes found in the retina. One week after modeling, the latent period of a-waves and b waves of FERG were delayed （ P 〈0.01 ）, but amplitude of a-waves and b waves dldnt change. Six weeks after modeling, these amplitudes started to de- dine in diabetic rats （ P 〈0. 01 }. Condusion The way of establishing diabetic rat model by intmperitoneal injection of STZ is simple and convenient, and the success rate is high. This medel is ideal and stable for diabetic retinopathy resear- cbes. The retinal function of early stage diabetic rat has changed before the visible changes of the retina happened.

关 键 词：糖尿病视网膜病变 动物模型 视网膜电图 电生理 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]