Yes相关蛋白在前列腺癌中的表达及意义  

Expression and significance of Yes-associated protein in prostate cancer

作　　者：李文宾[1] 王德林[1] 盛夏[1] 刘武江[2] 罗照[1] 

机构地区：[1]重庆医科大学附属第一医院泌尿外科,重庆400016 [2]北京大学第一医院泌尿外科、北京大学泌尿外科研究所,北京100034

出　　处：《重庆医科大学学报》2014年第1期5-8,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:30972999)

摘　　要：目的:探讨Yes相关蛋白(Yes-associated protein,YAP)在前列腺癌(prostate cancer,PCa)组织、癌旁(pericarcinoma,PC)组织和良性前列腺增生(benign prostatic hyperplasia,BPH)组织中的表达差异及其意义。方法:采用免疫组化技术检测32例PCa,15例PC和15例BPH中YAP的表达情况;采用Western blot技术检测15例PCa和相应PC中YAP的表达水平。结果:32例PCa中YAP阳性表达25例,阳性率为78.13%;15例PC中YAP阳性表达4例,阳性率为26.67%;15例BPH中未发现YAP阳性表达。组间差异有统计学意义(P=0.000);YAP的表达与PCa的临床分期、Gleason评分有关(分别为P=0.019;P=0.012)。临床分期越高,Gleason评分越高,YAP的阳性率越高。Western blot显示15例PCa中YAP表达(0.723±0.084)均高于相应PC(0.455±0.161),差异具有统计学意义(P=0.006)。结论:YAP在PCa中的表达明显高于非肿瘤组织,表现出明显的肿瘤特异性,有望成为PCa临床诊断和治疗的一个新指标。Objective ：To investigate the expression and clinical significance of Yes-associated protein（YAP） in tissues of prostate can- cer （PCa）, pericarcinoma （PC） and benign prostate hyperplasia （BPH）. Methods： Expression of YAP protein of 32 cases of PCa, 15 cases of PC and 15 cases of BPH was investigated by immunohistochemistry. Moreover,different expression of YAP protein of 15 cas- es of PCa and its corresponding PC tissues were confirmed by Western blot. Results：Rate of YAP positive staining（78.13%, 25/32） in PCa tissues was higher than that in PC tissues（26.67%,4/15） and YAP positive staining was not seen in BPH tissues. Staining differ- ences between each group were statistically significant（P=0.000）. Furthermore,different expression of YAP protein was relevant with clinical stage and degree of differentiation （P=0.019,P=0.012）. The higher the clinical stage, the higher the Gleason score and YAP positive staining rate. Western blot demonstrated that YAP protein expression was higher in 15 cases of PCa（0.723 ±0.084） than in corresponding PC tissues （0.455 ± 0.161）,with statistically significant differences （P=0.006）. Conclusions：Expression of YAP protein is significantly higher in PCa tissues than in non-tumorous tissues, demonstrating obvious tumor specificity in PCa. YAP may be a new target in the diagnosis and treatment of PCa.

关 键 词：前列腺癌 Yes相关蛋白 免疫组化 蛋白质免疫印迹 

分 类 号：R697.3[医药卫生—泌尿科学]