转录因子激活蛋白-2在Buerger’s病中的表达与意义  

Expression and significance of transcription factor activator protein-2 in Buerger's disease

作　　者：郑文涛[1] 李涛[1] 吴田芳 代远斌[1] 

机构地区：[1]重庆医科大学附属第一医院血管外科,重庆400016

出　　处：《重庆医科大学学报》2014年第1期47-50,共4页Journal of Chongqing Medical University

摘　　要：目的:观察转录因子激活蛋白-2(transcription factor activator protein-2,AP-2)在Buerger’s病中的表达与分布。方法:临床收集2011年1月至2012年3月共23例行腰交感神经节切除术Buerger’s病患者腰交感神经节标本,应用RT-PCR、Western blot方法检测AP-2在Buerger’s病组中基因和蛋白的表达变化,使用免疫组化的方法检测AP-2在Buerger’s病组中的表达分布规律,以观察在该组中的表达特点。临床收集12例正常病例标本以同样方法处理。结果:RT-PCR和Western blot结果显示,与正常对照组中相比,Buerger’s病组中AP-2基因和蛋白表达水平具有统计学差异(P<0.05),免疫组化显示病理组AP-2表达高于正常组,主要分布在细胞核。结论:Buerger’s病组中AP-2水平升高,表明AP-2在Buerger’s病的发生与发展过程中可能发挥了重要作用。Objective：To investigate the expression and distribution of transcription factor activator protein-2（AP-2） in Buerger＇s dis- ease. Methods：Lumbar sympathetic ganglia were sampled in a total 23 patients with Buerger＇s disease between January 2011 and March 2012. Gene and protein expression of AP-2 in the lumbar sympathetic ganglia neurons was observed by RT-PCR and Western blot. Distribution principles of AP-2 in the lumbar sympathetic ganglia neurons were observed by immunohistochemical methods. Twelve normal lumbar sympathetic ganglia were processed by the same way. Results：RT-PCR and Western blot results demonstrated that AP-2 gene and protein expression levels in Buerger＇s disease tissues were significantly different from those in normal tissues （P〈0.05）. Immunohistochemical result demonstrated that AP-2 expressions in Buerger＇s disease tissues were higher than those in nor- mal tissues,mainly distributed in the nucleus. Conclusions ：AP-2 level increases in patients with Buerger＇s disease ,indicating that AP-2 plays an important role in the occurrence and development of Buerger＇s disease.

关 键 词：Buerger’s病 转录因子激活蛋白-2 炎症因子 

分 类 号：R654.4[医药卫生—外科学]