细胞悬浮液介电谱检测与应用研究进展  

Progress of the technology and application of dielectric spectroscopy of cell suspensions

作　　者：丁冲[1] 刘玺[1] 商澎[1] 

机构地区：[1]西北工业大学生命学院特殊环境生物物理学研究所空间生物实验模拟技术重点实验室,西安710072

出　　处：《北京生物医学工程》2014年第1期94-100,共7页Beijing Biomedical Engineering

基　　金：国家自然科学基金(51147008);高等学校博士学科点专项科研基金(20126102110055)资助

摘　　要：细胞悬浮液介电谱是一种快速、实时、无损进行细胞物理特性定量研究的方法,在研究细胞构成和细胞生命活动过程中具有重要意义。细胞的结构和组成使其成为介电谱研究最适合的体系之一。细胞悬浮液介电谱随着检测频率的增加呈现α、β和γ三个典型的弛豫,对弛豫类型的解析有助于了解细胞组分和活动的信息。而细胞电参数模型的发展将细胞介电特性的研究深化到亚细胞层面,有助于了解更加细致的细胞组分和生命活动的信息。本文对细胞的介电行为,细胞悬浮液介电谱的检测、解析以及应用进行简要综述。The dielectric spectroscopy for cell suspension is a rapid, real-time, nondestructive method for the quantitative research on the physical properties of eel.l, which is of great significance in the process of studying cell components and cell life activities. Cell is one of the most suitable systems for dielectric spectroscopy research because of the structure and composition. With the frequency increased, the dielectric spectroscopy of cell suspension shows three main dielectric relaxation known as the α, β and γ dispersion. The information of cell components and activities could be achieved by analyzing these dielectric relaxations. Moreover, the development of the cell model for dielectric parameters helps the research of dielectric spectroscopy for cell suspension deepen to the subcellular level, with gaining more detailed information for cell components and activities. In this review article, the dielectric behavior of cells, the detection, analysis and application of dielectric spectroscopy for cell suspension are summarized briefly.

关 键 词：细胞悬浮液 介电谱 介电弛豫 单壳模型 双壳模型 

分 类 号：R318.04[医药卫生—生物医学工程]