ERα及ERβ在HBV相关肝细胞癌中的表达及临床意义  

Expression of estrogen receptors α and β in hepatitis B virus-related hepatocellular carcinoma and clinical significance

作　　者：罗晓丽[1] 谭文婷[1] 周媛[2] 但芸婕[1] 刘娜娜[1] 郭红[2] 邓国宏[1] 

机构地区：[1]第三军医大学西南医院全军感染病研究所,感染病研究重庆市重点实验室,重庆400038 [2]第三军医大学新桥医院消化内科,重庆400037

出　　处：《第三军医大学学报》2014年第6期564-567,共4页Journal of Third Military Medical University

基　　金：国家重点基础研究发展计划(973计划;2011CB512106);国家自然科学基金(81071694);第三军医大学临床基金重大专项(2012XLC05)~~

摘　　要：目的探讨雌激素受体（estrogen receptor，ER）α及β在乙型肝炎病毒（hepatitis Bvrius，HBV）相关肝细胞癌（hepatocellular carcinoma，HCC）组织中的表达水平及其与临床病理特征的关系。方法收集组织学病理诊断为肝细胞癌，且为慢性乙型肝炎的28例患者的肝癌组织及其相关临床资料，采用免疫组化EnVision法检测ER0l和ERβ的表达，分析其与临床病理特点的关系。结果ERα及ERβ在HBV相关HCC组织中表达以细胞质为主，其阳性表达率分别为35．7％（10／28）和46．4％（13／28）。其中ERa的阳性表达与患者肿瘤大小及TNM分期有关，肿瘤直径≤5cm的患者表达率高于肿瘤直径〉5cm的患者（60．0％US7．7％，P=0．006），TNM分期I-Ⅱ期患者的表达率明显高于Ⅲ-Ⅳ期的患者（60．0％ us 16．7％，P=0．011）。ERβ的表达与患者的性别、年龄、肿瘤大小、肿瘤分化程度、TNM分期、结节多少及血清AFP水平无关。结论ER在HBV相关HCC中存在差异表达，且以细胞质表达为主，其与HCC的临床病理特点有关。Objective To evaluate estrogen receptors （ERs） α and β expression in hepatitis B virus （HBV）-related hepatocellular carcinoma （HCC） in China and their relationship with clinical and pathological characteristics. Methods Expression of ERα and ERβ in 28 cases of HBV-related HCC was detected by immunohistochemistry technique EnVision method. ERα and ERβ relationship with the clinical and pathological characteristics of HBV-related HCC was analyzed statistically. Results Both ERet and ERI3 were expressed in the cytoplasm. The positive expression rate of ERα and ERβ was 35.7% （ 10/28 ） and 46.4% （ 13/28 ）, respectively. There was correlation between ERα expression and tumor diameter and TNM stage. Higher ERα expression was found in tumor with diameter ≤5 cm than in tumor with diameter 〉 5 cm （60.0% vs 7.7%, P =0. 006）, as well as in tumor with TNM stage I to II than in tumor with TNM stage 111 to 1V （60.0% vs 16.7%, P =0. 011 ）. ERβ expression displayed no significant association with age, sex, tumor size, patholog-ic subtype, TNM stage, nodule number and serum AFP level. Conclusion ERs expression has differences in HBV-related HCC and is associated with the clinical pathological characteristics of HCC.

关 键 词：肝细胞癌 肝炎病毒 乙型 雌激素受体Α 雌激素受体Β 

分 类 号：R373.21[医药卫生—病原生物学] R730.23[医药卫生—基础医学]