壮骨1号方对大鼠骨性关节炎的抗损伤作用研究  

Anti-injury Effect of Zhuanggu Yihao Fang on Osteoarthritis

作　　者：张少卓[1] 钟文婕[1] 周昆[1] 田文全[1] 高文远[2] 柳占彪[1] 

机构地区：[1]天津中医药大学,天津300193 [2]天津大学药物科学与技术学院,天津300072

出　　处：《中国实验方剂学杂志》2014年第6期132-135,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2011ZX09201-201-21)

摘　　要：目的:观察壮骨1号方对骨性关节炎的抗损伤作用。方法:选SD大鼠,以泊洛沙姆载药体加载木瓜蛋白酶制成温敏型原位凝胶控释剂,按照0.05 mL(1.6 U)/关节剂量,双侧膝关节注射,50℃环境下作用2 h复制大鼠骨性关节炎模型。实验分为正常对照、模型对照组(等容蒸馏水)、壮骨关节丸(1.10 g·kg-1)组与壮骨1号方(2.20,1.10,0.55 g·kg-1)治疗组,氨基葡糖(0.03 g·kg-1)阳性药对照组,ig每天1次,连续治疗8周。用酶联免疫吸附(ELISA)法测定关节液中基质金属蛋白酶-3(MMP-3)及其组织金属蛋白酶抑制因子-1(TIMP-1)含量,计算MMP-3/TIMP-1;观察关节软骨病理组织学变化并进行图像分析。结果:治疗8周后,模型组大鼠关节液中MMP-3/TIMP-1为0.092±0.010,与正常组0.062±0.011差异有统计学意义(P<0.05),用壮骨1号方2.20,1.10,0.55 g·kg-1剂量治疗8周后,MMP-3/TIMP-1明显降低,分别为0.060±0.016,0.060±0.028,0.057±0.027,与模型组比差异有统计学意义(P<0.05或P<0.01)。病理图像分析显示模型组大鼠软骨坏死面积为(37 734±11 504)μm2,与正常组(0.00±0.00)μm2比较差异有统计学意义(P<0.01),壮骨1号方2.20,1.10 g·kg-1治疗8周后,关节软骨坏死面积为(26 601±6 506),(27 061±10 425)μm2,与模型组比较差异有统计学意义(P<0.05)。结论:壮骨1号方具有一定的抗软骨损伤作用,能延缓骨性关节炎的病程进展。Objective：To explore the anti-injury effect of Zhuanggu Yihao Fang on osteoarthritis （OA）.Method：The OA rat model was induced by sustained releasing agent （4％ Papain and poloxamer）.0.05 mL of the sustained releasing agent （4％ Papain and poloxamer） was injected into both knee joint compartments of male SD rats to injury cartilage for a period of 2 h at 50 ℃.Besides normal group,the rats with osteoarthritis were randomly divided into 6 groups：model group （isovolumic distilled water）,glucosamine group （0.03 g ·kg-1）,Zhuanggu Guanjie pill （1.10 g·kg-1） group and Zhuanggu Yihao Fang （2.20,1.10,0.55 g ·kg-1） groups.The rats with osteoarthritis received daily oral treatments of drugs for 8 weeks.The normal group and the model group received daily oral treatments of isovolumic distilled water for 8 weeks.The contents of matrix metalloproteinases-3 （MMP-3） and tissue inhibitar of metalloproteinases-1 （TIMP-1） in synovial fluid were detected by enzyme-linked immunosorbent assay （ELISA）.The changes in cartilages were evaluated by histopathology and morphometry.Result：MMP-3/TIMP-1 level in normal group was 0.062 ± 0.011,compared with the normal group,MMP-3/TIMP-1 level in the model group was significantly increased to 0.092 ± 0.010 （P ＜0.05）.After 8 weeks＇ treatment,the MMP-3/TIMP-1 level in Zhuanggu Yihao Fang （2.20,1.10,0.55 g ·kg-1） groups was 0.060 ± 0.016,0.060 ± 0.028,0.057 ± 0.027,respectively.Compared with the model group,Zhuanggu Yihao Fang （2.20,1.10,0.55 g·kg-1） treatments significantly reduced the MMP-3/TIMP-1 level （P ＜ 0.05,P ＜ 0.01）.Compared with the normal group,the area of cartilage necrosis in the model group was significantly increased to （37 734 ± 11 504） μm2 （P ＜0.01）,Zhuanggu Yihao Fang （2.20,1.10 g ·kg-1） treatments significantly reduced the areas of cartilage necrosis in OA rats,which was （26 601 ± 6 506）,（27 061 ± 10 425） μm2,respectively （P ＜ 0.05）.Conclusion：Zhuanggu Yihao Fan

关 键 词：壮骨1号方 骨性关节炎 抗损伤作用 

分 类 号：R285.5[医药卫生—中药学]