黄芪药渣发酵产物富硒酵母对免疫低下糖尿病大鼠的影响  

Effect of Selenium Yeast (Astragalus membranaceus Fermentation Product) on Diabetes Rat with Immune-suppression

作　　者：刘必旺[1] 许凯霞[1] 郭羽[1] 王一捷[1] 李敏[1] 解冬[1] 

机构地区：[1]山西中医学院,太原030024

出　　处：《中国实验方剂学杂志》2014年第6期136-139,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81102553);国家"十二五"科技支撑项目(2011BAI07B01;2013BAI05B08);山西省科技攻关项目(20110313001-2);山西省特色重点学科中医学

摘　　要：目的:研究黄芪药渣发酵产物富硒(Se)酵母对免疫低下糖尿病大鼠的影响。方法:80只SD大鼠,10只留为正常对照组,70只ip链脲佐菌素55 mg·kg-11次,环磷酰胺100 mg·kg-13次,造免疫低下糖尿病模型动物,造模成功的动物随机分成5组(每组10只):免疫低下糖尿病模型组、盐酸二甲双胍片0.1 g·kg-1阳性对照组、酵母溶液0.5 g·kg-1组、黄芪提取液5 g·kg-1组、富Se酵母0.5 g·kg-1组。各组固定饲料,自由饮水。分别给予相应药物ig,持续给药4周;每周称重。给药4周后,腹主动脉取血,分离血清,测定空腹血糖(FBG)、血常规[红细胞(RBC)、血红蛋白(HB)、白细胞(WBC)]、免疫球蛋白(IgG)、白介素-2(IL-2)、胰岛素(INS)水平,并进行胰腺组织形态学观察及胰岛素样生长因子(IGF)的检测。结果:模型组FBG与正常组FBG比较有显著性差异(P<0.01),模型组INS,WBC,IgG,IL-2与正常组相应指标比较有统计学差异(P<0.05);富Se酵母组FBG,INS,WBC,IgG,IL-2与模型组相应指标比较有统计学差异(P<0.05)。富Se酵母组与模型对照组比较其他各项指标也有所改善。结论:富Se酵母可以降低FBG和提高INS水平,同时可提高WBC,IgG,IL-2等免疫指标,富Se酵母可使免疫力低下糖尿病模型动物血糖降低,免疫力增强。采用发酵的技术从中药水煎液后的药渣中提取微量元素是一个药材深入开发使用的良好途径。Objective：To study effect of selenium （Se） yeast （Astragalus membranaceus fermentation product） on diabetes rats with immune-suppression.Method：Eighty SD rats,10 to the normal control group,70 rats to establish experimental immune-suppression and diabetic rat model and watch Se yeast on the effects of streptozotocin diabetes and cyclophosphamide immunocompromised rat.The successful model of the animal were randomly divided into 5 groups （n =10 each group）：diabetic model with immune-suppression group,oxytetracycline tablets control group （0.1 g ·kg-1）,yeast solution group （0.5 g ·kg-1）,A.membranaceus extract group （5 g ·kg-1）,Se yeast group （0.5 g ·kg-1）.Each group fixed feed,free drinking water.Four weeks after the last to give the corresponding drug,abdominal aortic blood,separation of serum,fasting blood glucose （FBG）,blood [red blood cell （RBC）,hemoglobin （HB）,white blood cell （WBC）],immunoglobulin （IgG）,interleukin-2 （IL-2）,insulin （INS） level,and pancreatic histological observation and insulin like growth factor （IGF） were detected.Result：In the model group,FBG were significant difference to normal group FBG （P ＜ 0.01）,model group INS,WBC,IgG,IL-2 were statistically significant difference to normal group corresponding index （P ＜ 0.05）.Se yeast group FBG,INS,WBC,IgG,IL-2 have statistical difference to the corresponding index in the model group （P ＜0.05）.Se yeast group with compared other indicators have also improved with the model group.Conclusion：Se yeast can reduce FBG and increase the level of INS,also can enhance the WBC,IgG,IL-2 and other immune parameters,Se yeast can make the immunity of animal models of diabetes mellitus reduce blood sugar,enhance immunity.Extracted from the dregs of decoction of trace elements is a good way to use the development of a drug by fermentation technology.

关 键 词：富Se酵母 糖尿病 免疫低下 黄芪 药渣 

分 类 号：R285.5[医药卫生—中药学]