脉血康胶囊对早期AS大鼠脂代谢及血管壁LOX-1表达的影响  

Influence of Maixuekang Capsule on Lipid Metabolism and Expression of Lectin Like Oxidized Low Density Lipoprotein Receptor-1 in Early Atherosclerosis Rats

作　　者：胡耀红[1] 杨乔[2] 高丽娟[1] 

机构地区：[1]天津医科大学研究生院,天津300070 [2]天津医科大学总医院中医科,天津300052

出　　处：《中国实验方剂学杂志》2014年第6期157-161,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：天津市中医药管理局中医;中西医结合课题

摘　　要：目的:探讨脉血康胶囊对动脉粥样硬化(AS)大鼠脂质代谢及血凝素样氧化低密度脂蛋白受体1(LOX-1)表达的影响及其可能机制。方法:将32只健康雄性SD大鼠随机分正常对照组,模型组,脉血康高、低剂量组(1.5,0.3 g·kg-1)。采用高脂饮食+腹腔注射维生素D3的方法建立大鼠早期AS模型,治疗组在高脂饮食的同时每天给予相应药物灌胃,连续喂养10周。检测血脂[总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)]、同型半胱氨酸(HCY)、过氧化氢酶(CAT),用ELISA法检测血清氧化低密度脂蛋白(ox-LDL)及主动脉壁血凝素样氧化低密度脂蛋白受体1(LOX-1)的表达,取大鼠胸主动脉作病理学观察。结果:与正常对照组相比,模型组血清TC,TG,LDL-C,HCY显著升高(P<0.05或P<0.01),HDL-C,CAT显著降低(P<0.01),ox-LDL和LOX-1表达(34.17±13.4),(1.58±0.08)μg·L-1较正常对照组(20.37±2.5),(1.44±0.08)μg·L-1明显升高(P<0.05或P<0.01);脉血康胶囊可以明显降低TC,TG,LDL-C,HCY,升高HDL-C,CAT(P<0.05或P<0.01),下调ox-LDL(28.92±4.5,21.81±3.9)μg·L-1和LOX-1(1.45±0.07,1.39±0.09)μg·L-1的表达(P<0.05或P<0.01)。胸主动脉石蜡切片HE染色显示模型组内、中膜增厚,弹力纤维增生,平滑肌排列紊乱;与之相比脉血康各组内、中膜增厚不明显,弹力纤维轻度增粗,平滑肌细胞排列较整齐。结论:脉血康胶囊通过调节脂质代谢,减轻氧化损伤,降低血清ox-LDL水平及下调其特异性受体LOX-1的表达等环节,保护血管内皮,抑制动脉粥样硬化的发生发展。Objective：To investigate the influence and possible mechanism of Maixuekang capsule on lipid metabolism and expression of lectin like oxidized low density lipoprotein receptor-1 （LOX-1） in early atherosclerosis rats.Method：Thirty-two healthy male SD rats were randomly divided into normal control group,model control group,high and low dose groups （1.5,0.3 g ·kg-1 ·d-1） of Maixuekang capsule.Model control group and Maixuekang capsule groups were intraperitoneally injected by 6 × 105 unit vitamin D3,and corresponding medicines were ig administrated every day on the basis of high-fat diet for 10 successive weeks.Levels of blood lipid profile,homocysteine （HCY）,catalase （CAT） were observed.The expression of oxidized low density lipoprotein （ox-LDL） in serum and LOX-1 in aortic wall were detected by ELISA.The pathology of thoracic aortas were observed.Result：In model control,the levels of TC,TG,LDL-C,HCY were increased than normal control （P ＜ 0.05 or P ＜ 0.01）,HDL-C and CAT were decreased （P ＜ 0.01）.The expression of ox-LDL and LOX-1 was significantly increased （P ＜0.05 or P ＜0.01） in model control （34.17 ± 13.4）,（1.58 ±0.08） μg ·L-1than the normal control （20.37 ± 2.5）,（1.44 ± 0.08） μg ·L-1.Maixuekang capsule decreased the levels ofTC,TG,LDL-C,HCY and increased the levels of HDL-C and CAT （P ＜0.05 or P ＜0.01）; the expression ofox-LDL （28.92±4.5）,（21.81±3.9） μg· L-1and LOX-1 （1.45±0.07）,（1.39±0.09） μg · L-1 was attenuated in all the treatment groups （P ＜ 0.05 or P ＜ 0.01）.In model control,intima-media thickness,elastic fiber proliferation and disordered arrangement of smooth muscle cells were found in thoracic aortas by paraffin section and Hematoxylin staining.By contrast,intima-media thickness were not obvious,elastic fiber was mild wided and arrangement of smooth muscle cells in Maixuekang capsule group.Conclusion：The Maixuekang capsule can protect vascular endothelial cell and inhibit atherosclerosi

关 键 词：脉血康胶囊 早期动脉粥样硬化模型 过氧化氢酶 同型半胱氨酸 血凝素样氧化低密度脂蛋白受体1 

分 类 号：R285.5[医药卫生—中药学]