补肾活血方对兔膝骨性关节炎的治疗作用研究  

Effect of Bushen Huoxue Formulae on Knee Osteoarthritis in Rabbits

作　　者：于娟[1] 李莎莎[1] 叶莹仪[1] 葛鸿庆[1] 陈文治[1] 邓晓强[1] 余宇峰[1] 

机构地区：[1]广东省中医院,广州510120

出　　处：《中国实验方剂学杂志》2014年第6期171-174,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技厅项目(2008B030301361)

摘　　要：目的:通过观察补肾活血方对Hulth法复制兔膝骨性关节炎模型中软骨组织结构的改变及基质金属蛋白酶-2(MMP-2)、白细胞介素-1β(IL-1β)、肿瘤坏死因子(TNF-α)浓度的影响,探讨其对关节炎的治疗作用及机制。方法:48只新西兰兔,6只为正常对照,42只用Hulth法复制骨关节炎模型,4周后在模型中随机处死6只动物取材光镜下观察,造模成功后将其余36只随机分为治疗组和模型组,治疗组以补肾活血方15 g·kg-1·d-1ig,模型组予同体积生理盐水。给药后的第4,6,8周分别在2组随机处死6只动物,取48只动物股骨髁软骨做石蜡切片行HE染色、观察软骨细胞形态,用ELISA试剂盒检测血清中IL-1β,TNF-α及关节液中MMP-2。结果:与正常组比较,模型组IL-1在第4,6,8周显著升高(P<0.01),MMP-2,TNF-α在第4,6周显著升高(P<0.01)。与模型组相比,Mankin's分数在第8周治疗组显著降低(P<0.001);IL-1β在第8周治疗组血清中显著降低(P<0.05);MMP-2在第6周关节液中显著降低(P<0.01);TNF-α在第6周血清中显著降低(P<0.05)。结论:补肾活血方可降低IL-1β含量,在关节炎早期可降低MMP-2和TNF-α,并可改善软骨组织形态从而保护关节软骨。Objective：To investigate pathological changes of the cartilage and concentrations of interleukin-1β （IL-1β）,matrix metalloproteinases （MMPs） and blood tumor necrosis factor （TNF-α） by Bushen Huoxue formulae in treating rabbit knee osteoarthritis model copied by Hulth surgery.Method：Forty-eight rabbits were randomly divided control group with 6 rabbits,and model group are forty-two rabbits.The osteoarthritis model was established by Hulth surgery,among them 6 animals were randomly killed after modeling 4 weeks for microscope observation,and the remaining 36 rabbits were randomly divided into treatment group and model group,treatment group was given 15 g ·kg-1 Bushen Huoxue,the model group was given the same dose of saline.After 4,6,8 weeks,6 animals were killed in each groups respectively to collect the medial femoral condyle to observe chondrocytes morphology by HE staining,meanwhile,the concentrations of IL-1β,MMP-2,TNF-α were assayed.Result：Compared with the normal group,in model group,concentration of IL-1β was significantly higher at 4,6,8 weeks （P ＜ 0.01） ; the concentration of MMP-2 and TNF-α was significantly higher in the 4th and 6th weeks （P ＜ 0.01）.Compared with the model group,the Mankin＇s score in treatment group was much significantly lower （P ＜ 0.001） in 8 week.the serum concentration of IL-1β significantly was lower （P ＜ 0.05） in 8 week,the concentration of MMP-2 in joint fluid was significantly lower （P ＜ 0.01） in 6 week,the serum concentrations of TNF-α was significantly lower （P ＜ 0.05） in 6 week.Conclusion：Bushen Huoxue treatment can reduce the concentrations of IL-1β,MMP-2 and TNF-α,it can change the form of cartilage tissue so as to protect the articular cartilage in arthritis.

关 键 词：补肾活血方 膝骨性关节炎 炎症细胞因子 

分 类 号：R285.5[医药卫生—中药学]