玉郎伞多糖抗鸭乙型肝炎病毒作用  

Yulangsan Polysaccharide Affects Anti-Duck Hepatitis B Virus in Brown Duck

作　　者：郭雪峰[1] 罗秀[1] 何巧玲[1] 李秀菊[1] 明建军[1] 黄仁彬[1] 张士军[1] 

机构地区：[1]广西医科大学药理学教研室,南宁530021

出　　处：《中国实验方剂学杂志》2014年第6期175-178,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西地方特有中药有效成分与机制研究(桂教科研201202ZD023);广西地方性高发疾病防治重点实验室开放课题(桂科能0842009-K4)

摘　　要：目的:研究玉郎伞多糖(YLSP)抗鸭乙型肝炎病毒(duck hepatitis B virus,DHBV)的作用。方法:取1日龄广西麻鸭60只,在麻鸭颈静脉注射0.2 mL强阳性DHBV DNA鸭血清,13 d后分别从其颈静脉采血0.5 mL,分离血清,用普通PCR法筛选出DHBV感染强阳性鸭。然后,将50只DHBV DNA阳性麻鸭随机分为5组:YLSP高、中、低剂量组(5,2.5,1.25 g·kg-1);阿昔洛韦阳性对照组(0.1 mg·kg-1)和模型组。所有给药组均与模型组比较,以明确玉郞伞多糖对DHBV DNA的作用,所以未设置正常组。于给药前(T0),给药7 d(T7),14 d(T14)及停药后3 d(P3)采血,用实时荧光定量PCR法检测鸭血清中DHBV DNA的含量。HE染色观察肝损伤程度。结果:与模型组相比,YLSP各剂量组及阳性对照组在用药后血清中DHBV DNA的含量显著降低,停药后,阳性对照组及YLSP中、低剂量组DHBV DNA含量均有反跳现象。HE染色结果显示YLSP能减轻鸭肝组织的病理损伤程度。结论:YLSP对DHBV具有抑制作用同时对肝损伤具有保护作用。Objective：To investigatewhether Yulangsan Polysaccharide （YLSP） affects the anti-Duck Hepatitis B Virus （anti-DHBV） in brown duck.Method：Sixty one-day-old ducks were selected.After 13 days,positive ducks infected 0.2 mL serums of DHBV DNA were detected by PCR,and were randomly divided into five groups：YLSP high-,middle-,low-dose groups （5,2.5,1.25 g·kg-1）,aciclovir （ACV）group （0.1 mg· kg-1） and model group with 10 brown ducksin every group.The serums were respectively collected before administration,7 and 14 days after administration and 3 day after withdrawal.The DHBV DNA was detected by fluorescence quantitative （FQ）-PCR assay.The extent of liver injury was observed by HE staining.Result：Compared with model group,the serum content of DHBV DNA in ACV group and YLSP 5,2.5,1.25 g · kg-1 groups was significantly decreased.The content of DHBV DNA in ACV group,middle-does group and low-dose groups showed a rebound phenomenon after withdrawal.The results indicatedthat YLSP had significant protective effects against the injury of ducks＇ liver.Conclusion：YLSP not only has an inhibited effect to DHBV,but also has a protective effect on injury of liver.

关 键 词：玉郎伞多糖 鸭乙型肝炎病毒 实时荧光定量PCR 

分 类 号：R285.5[医药卫生—中药学]