MUC13腹泻抗性纯合杜洛克种群的选育研究  

Selection of Duroc Breeds Homozygous for MUC13 Diarrhea-resistant Allele

作　　者：阮国荣[1] 何晓芳[2] 黄晶[1] 肖石军[3] 陈金雄[1] 李军山 刘亚轩 孙耀华 陈福珍 林国忠 

机构地区：[1]福建农业职业技术学院,福建福州350119 [2]上海农业科学院畜牧兽医研究所,上海201106 [3]江西农业大学生物技术国家重点实验室培育基地,江西南昌330045 [4]福建省厦门国寿种猪开发有限公司,福建厦门350021 [5]福建省光华百斯特生态农牧有限公司,福建尤溪365100

出　　处：《江西农业大学学报》2014年第1期174-180,共7页Acta Agriculturae Universitatis Jiangxiensis

基　　金：福建省科技计划重点项目(2010N0012);福建省农业跨越计划项目(2013KY05)

摘　　要：MUC13是控制仔猪大肠杆菌F4ac易感或抗性的主效基因。本研究利用分子育种与常规育种技术、闭锁继代与开放选育相结合的综合方法,对250头杜洛克育种核心群实施连续3个世代的基因检测和性能测定,筛选出MUC13抗性纯合基因型种群。经测试,在相同饲养管理条件下,哺乳期的同胎次20窝育种群母猪与20窝扩繁群母猪相比,断奶前仔猪腹泻发生率分别为8.7%和18.4%(P<0.01),但断奶成活率两组间差异未达到显著水平(P>0.05);对育种群3个世代的测定选育结果分析发现,第一和第三世代间达100 kg体质量日龄差异不显著(P>0.05)、但活体背膘厚相比减少1.46 mm(P<0.05),同时还发现,3个世代中MUC13不同基因型个体间生长速度和活体背膘差异均不显著(P>0.05)。综上说明,MUC13基因抗性纯合有利于防止仔猪腹泻的发生并能有效降低活体背膘,但对生长发育没有明显影响。MUC13 is a major gene to control ETEC F4ac adhesion that causes diarrhea in piglets .In the present study ,the MUC13 resistant allele was determined and its performance traits were recorded in a Duroc nucleus population of 250 individuals through three successive generations using molecular breeding and con-ventional breeding techniques .After the three-generation selection ,20 litters of breeding sows and 20 litters ofmultiplier sows at the same litter during lactation were observed under the same feeding and management con-ditions in the experiment.As a result,the diarrhea incidence rate was 8.7%and 18.4%in the breeding sows and propagation sows before weaning ,respectively.Of note,this difference was significant （P〈0.01） in two groups while no significant difference was found in survival rate between these two groups when weaned .Fur-thermore,no significant difference in the date when the weight was up to 100 kg after 3 generation’s measure-ment and selection was found between the first generation and third generation in the breeding population .On the contrary,live backfat thickness was reduced by 1.46 mm and showed a significant difference between the two generations （P〈0.05）.In addition,no significant differences were found in growth rate and live backfat thickness across the different individuals of genotype .In conclusion ,the MUC13-resistant allele can confer re-sistance to diarrhea in piglets and reduce live backfat thickness in adult pigs whereas has no significant effect on growth and development .

关 键 词：杜洛克 MUC13 选育 

分 类 号：S828.2[农业科学—畜牧学]