6-苄基腺嘌呤延缓水稻衰亡效应的蛋白质组学分析  

Proteomics Analysis of Mechanism of 6-benzyl Adenine Deferring Contabescence of Rice

作　　者：邵彩虹[1] 唐秀英[1] 李明心[2] 李瑶[1] 王萍[1] 陈金[1] 谢金水[1] 

机构地区：[1]江西省农业科学院土壤肥料与资源环境研究所,江西南昌330200 [2]江西经济管理干部学院,江西南昌330200

出　　处：《华北农学报》2014年第1期14-19,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家科技支撑计划项目(2011BAD16B04;2013BAD07B00;2012BAD04B00);江西省自然科学基金项目(20114BAB214010;20132BAB204034)

摘　　要：以苗期杂交水稻威优916为试验材料,分别以蒸馏水和8 mg/L 6-苄基腺嘌呤溶液培养,通过对功能叶片蛋白质组差异表达分析,以揭示养分胁迫下6-苄基腺嘌呤延缓水稻衰亡的效应及其机理。叶片全蛋白质经双向电泳分离,获得了22个响应6-苄基腺嘌呤处理的蛋白质,经质谱分析,其中15个功能得到鉴定,涉及光合作用、呼吸作用、激素代谢及逆境反应等多个代谢途径。分析结果显示,养分胁迫下,6-BA诱导了叶片中光合蛋白质、呼吸代谢相关蛋白质的表达量上调,减少衰亡发生密切相关蛋白质的积累,从而提高叶片光合能力,促进呼吸,满足植株生长对物质及能量的需求,延缓植株衰亡。In order to reveal the mechanism of 6-benzyl adenine deferring contabescence of rice,the hybrid rice Weiyou 916 seedlings were cultivated with distilled water and 6-benzyl adenine（8 mg/L） ,respectively,and the pro-teins expression profile of functional leaves were investigated by using the approach of proteomics. Proteins were ex-tracted from rice functional leaf and separated by two-dimensional gel electrophoresis,then 22 differential expression proteins that induced by 6-benzyl adenine were obtained,among which 15 proteins were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry, and categorized into 4 groups of functions including photosynthesis,respiration,hormone metabolism and stress response. Results showed that under nutrient stress con-dition,6-benzyl adenine had benifical effect on rice plant：the expression abundance of proteins associated with pho-tosynthesis and respiration increased,and proteins associated with contabescence were down-regulated in expression abundance. Thereby,application of 6-benzyl adenine chould enhanced the photosynthesis and respiration directly, provided the material and energy for plant growth and defered rice contabescence.

关 键 词：水稻 6-苄基腺嘌呤 衰亡 蛋白质组学 

分 类 号：S312.03[农业科学—作物栽培与耕作技术] Q78[农业科学—农艺学]