两种蚜虫携带百合无症病毒、烟草花叶病毒的基因芯片检测  

Detection of Two Kinds of Aphids Carried LSV,TMV with DNA Microarry

作　　者：贾慧[1] 郑洁[2] 孟庆江[1] 曹志艳[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071000 [2]河北农业大学科技处,河北保定071000

出　　处：《华北农学报》2014年第1期20-24,共5页Acta Agriculturae Boreali-Sinica

基　　金：北京市自然科学基金项目(5043026)

摘　　要：蚜虫作为病毒的传播介体为害植物造成的经济损失远远超过蚜虫本身,因此急需建立快速、准确、灵敏的蚜虫带毒检测方法。根据百合无症病毒(LSV)和烟草花叶病毒(TMV)外壳蛋白基因保守序列设计引物与探针,制备基因芯片;用Cy3标记下游引物,提取饲毒(LSV和TMV)棉蚜、桃蚜总RNA,RT-PCR扩增产物与芯片杂交,荧光扫描仪检测并分析信号。结果表明,该芯片可以同时检测上述2种蚜虫的带毒情况,此方法有较好的特异性和重复性,能快速、准确地对介体蚜虫的带毒情况进行检测。Aphid transmission of the virus as a mediator harm plants far more than economic losses caused by aphids themselves. So,rapid,sensitive and specific method,by which viruses carried by aphids can be detected sim-ultaneously,is needed urgently. In this study,according to the Lily symptomless virus （ LSV） and the Tobacco mosa-ic virus （ TMV） coat protein gene sequence,primers and probes were designed,gene chip was prepared. The down-stream primers were labeled by Cy3 . The total RNA was extracted from Aphis gossypii and Myzus perdicae raised with lily infected by LSV and TMV respectively. The product amplified by RT-PCR using the labeled primers was hybrid-ized with the gene chip. The hybridization signal was detected and analyzed by a fluorescence scanner. The result showed that the chip can simultaneously detect both aphids infected cases,this method has good specificity and re-producibility,fast and accurate on the mediator infected aphids were detected.

关 键 词：棉蚜 桃蚜 百合无症病毒 烟草花叶病毒 基因芯片 检测 

分 类 号：S432.4[农业科学—植物病理学] Q78[农业科学—农业昆虫与害虫防治]