利用VIGS技术分析野生茄Ve基因的抗黄萎病功能  

The Function Confirmation of Ve Gene Homolog in S. linnaeanum Related to Verticillium Wilt Resistance by VIGS

作　　者：刘军[1] 周晓慧[1] 冯翠[1] 武文[2] 庄勇[1] 

机构地区：[1]江苏省农业科学院蔬菜研究所,江苏南京210014 [2]中南林业科技大学风景园林学院,湖南长沙410004

出　　处：《华北农学报》2014年第1期217-221,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31101542);国家高技术研究发展计划项目(2012AA100103);江苏省自然科学基金项目(BK2011675);江苏省农业科技自主创新资金项目(CX(13)2003)

摘　　要：黄萎病是影响茄子生产的一种重要土传性病害,挖掘利用抗病基因对茄子抗黄萎病育种具有重要的意义。Ve类基因是已知的植物抗黄萎病基因之一。采用同源法从抗黄萎病野生茄中分离Ve类基因的同源序列,构建重组载体TRV2-SlVe,经农杆菌介导侵染植株并接种黄萎病病菌,结合表型观察和半定量RT-PCR技术对结果进行了分析。结果发现,TRV2-SlVe成功地抑制了野生茄中Ve基因的表达,接种植株表现出明显的黄萎病症状。以上结果表明,野生茄中的Ve类基因具有抗黄萎病的特性。Verticillium wilt is one of the most serious soil-borne diseases of eggplant. The exploration and utili-zation of Verticillium wilt resistant gene will play an important role for breeding disease resistant eggplants. Ve type genes have been confirmed to confer resistance to Verticillium wilt in some plant species. In this study,Ve homolo-gous sequence was isolated from a Verticillium wilt resistant wild eggplant S. linnaeanum,and it was used to con-struct a recombinant vector TRV2-SlVe. The seedlings were inoculated with TRV2-SlVe mediated by Agrobacterium and followed Verticillium dahlia. The results showed that the infected plants expressed symptoms of Verticillium wilt, and the expression of Ve gene was down-regulated in leaf,stem and root by semi-quantitative RT-PCR. Above results indicate that the Ve gene homolog in S. linnaeanum confers resistance to Verticillium wilt.

关 键 词：野生茄 VIGS 黄萎病 Ve基因 

分 类 号：S432.4[农业科学—植物病理学]