高效液相色谱法测定人脑脊液中比阿培南的浓度及临床应用  

Determination of biapenem in human cerebrospinal fluid by HPLC and its clinical application

作　　者：李明艳[1] 黄志伟[1] 吴洪文[1] 全香丽 

机构地区：[1]广西医科大学第四附属医院,广西柳州545005 [2]桂林医学院,广西桂林541004

出　　处：《中国医院药学杂志》2014年第5期364-366,共3页Chinese Journal of Hospital Pharmacy

基　　金：广西科学研究与技术开发计划项目(编号:桂科攻1140003A-43)

摘　　要：目的：建立一种高效液相色谱法测定人脑脊液中比阿培南浓度并应用于颅内感染的个体化治疗。方法：采用外标法，色谱柱为Shim-packVP-ODS柱，流动相为乙腈-0．1mol·L^-1醋酸钠缓冲液（2：98，pH4．6），流速为1．0ml·min^-1，检测波长300nm，柱温为30℃，并利用建立的方法对颅内感染患者进行药物浓度监测。结果：比阿培南在0．2～50mg·L^-1范围内线性良好（r=0．9994），定量下限为O．2mg·L^-1低、中、高（0．5，5，25mg·L^-1）质量浓度日内和El间RSD均≤10％，回收率在85％～110％之间，均满足生物样品测定要求。结论：本法简便快速准确，适用于比阿培南脑脊液浓度测定。OBJECTIVE To establish an HPLC method for determination of biapenem in human cerebrospinal and apply it to the therapy of intracranial infection. METHODS The chromatographical column was Shim-pack VP-ODS, the mobile phase consisted of acetonitile-0. 1 mol.L^-1 sodium acetate buffer solution （2：98, pH 4. 6）, the flow rate was 1.0 ml.min ^-1. The de tection wavelength was set at 300 nm, temperature was kept at 30 ℃. And biapenem concentrations in CSF of patients with in tracranial infection were monitored by this way. RESULTS Biapenem showed a good linear relation in the range of 0. 2 - 50 mg . L^-1 （r=0. 999 4） ,the limit of quantitation was 0. 2 mg.L^-1. At 0.5,5,25 mg.L ^-1 ,the intra-day and inter-day RSD were less than 10%, the recoveries were between 85% - 110%, all met the criteria for determination of biological samples. CONCLU SION The method is simple,rapid and precise,it is suitable for determination of biapenem in human cerebrospinal fluid.

关 键 词：高效液相色谱法 比阿培南 脑脊液浓度 

分 类 号：R942[医药卫生—药剂学]