醋酸曲安奈德柔性纳米脂质体的制备及瘢痕皮肤的透皮特性  

Preparation and transdermal characteristics in human scar skin of flexible nanoliposome containing triamcinolone acetonide

作　　者：邓王萍[1] 谭朝丹[1] 吴志宏[2] 张立超[1] 

机构地区：[1]上海市第七人民医院药剂科,上海200137 [2]上海市第七人民医院,烧伤科上海200137

出　　处：《中国医院药学杂志》2014年第5期367-370,共4页Chinese Journal of Hospital Pharmacy

基　　金：浦东新区卫生系统重点学科建设项目(编号:PWZXK2010-11);浦东新区卫生局科研项目(编号:PWRd2008-7)

摘　　要：目的:研究醋酸曲安奈德柔性纳米脂质体在人瘢痕皮肤中的透皮特性。方法:薄膜分散法制备柔性纳米脂质体,考察其形态、粒径和包封率;改良Franz扩散池和皮肤匀浆法观察体外及在体瘢痕透皮特性;HPLC法测定药物含量。结果:柔性纳米脂质体包封率(67.04±3.77)%。12 h体外累积透过量(7.84±0.99)μg·cm-2,分别是普通纳米脂质体和乳膏的2.20和4.43倍(P<0.05);12 h药物滞留量,分别是普通纳米脂质体和乳膏的2.23倍和7.24倍(P<0.05)。在体瘢痕试验12 h,柔性纳米脂质体表皮和真皮滞留量分别是乳膏的5.44和7.72倍(P<0.05)。结论:柔性纳米脂质体显著增加了醋酸曲安奈德在瘢痕皮肤中的药物透过量和滞留量,有望成为治疗瘢痕的新型局部给药制剂。OBJECTIVE To study the transdermal characteristics in human scar skin of flexible nanoliposome containing tri- amcinolone acetonide. METHODS Flexible nanoliposome was prepared by film dispersion method. The shape, particle size and the entrapment efficiency of nanoliposome were analyzed. The in vitro and in vivo transdermal characteristics in scar skin were investigated by modified Franz diffusion cell and the homogenization method, respectively. The content of triamcinolone ace tonide was measured by HPLC method, llESULTS The entrapment efficiency of the flexible nanoliposome was （67. 04 ± 3.77）%. The accumulated amount 12 h after the in vitro permeation experiment was （7. 84 ± 0. 99）μg.cm^-2 , which was 2. 20 and 4. 43 times of the high of the ordinary nanoliposome and the cream, respectively（P〈0. 05）. And the drug deposition with in human scar skin 12 h after the in vitro experiment, was 2. 23 and 7. 24 times of the high of the ordinary nanoliposome and the cream, respectively（P〈0. 05）. After 12 h the in vivo experiment in human scar skin, the drug deposition in epidermis and dermis of the flexible nanoliposome was 5.44 and 7. 72 times of the high of the cream（P〈0. 05）. CONCLUSION The flexible nanoliposome significantly improved the permeation of triamcinolone acetonide through and into skin, and might be a novel ve- hicle for the topical use in human scar skin tissue.

关 键 词：曲安奈德 柔性纳米脂质体 瘢痕皮肤 透皮特性 

分 类 号：R943[医药卫生—药剂学]