两种手术入路消融术建立兔椎间盘退变模型的比较研究  

Establishment of intervertebral disc degeneration model in rabbits by two different surgical approached

作　　者：周葳[1] 徐义春[1] 王其友[1] 韦家冬[1] 余灏涛[1] 赵飞[1] 

机构地区：[1]中山大学附属第三医院脊柱外科,广州510630

出　　处：《中华实验外科杂志》2014年第3期669-671,共3页Chinese Journal of Experimental Surgery

基　　金：广东省科技攻关计划资助项目（2009B030801095）;广东省科技攻关计划资助项目（2007A060300005）

摘　　要：目的 通过两种手术入路消融术建立兔椎间盘退变模型,探讨其优缺点及可能性.方法 新西兰大白兔24只,随机分为腹膜外入路及侧方入路组各12只.分别通过侧方及腹膜外的手术入路暴露椎间盘,穿刺L3/4～ L5/6椎间隙并消融30 s.术前及术后行CT、MRI检查及病理学检查.结果 两组内CT检查术后各时间点差异均有统计学意义（P＜0.01）.4周两组间差异有统计学意义（P＜0.01）.MRI T2矢状位两组均可见髓核信号降低,两组信号差异不明显.病理学检查可见两组均可见髓核丢失及椎间盘纤维化.结论 两种手术入路均可建立与人相似的退变模型,侧方入路早期退变更为明显.Objective To develop intervertebral disc （IVD） degeneration animal model by plasma radiofrequency ablation through two surgical approaches.Methods Twenty-four New Zealand white rabbitswere divided into Extreme-Lateral group and retroperitoneum group equally.L3/4-L5/6 IVDs were punctured and ablated for 30 s in two groups.CT imaging studies,MRI imaging studies and histological analysis were performed before operation,and 4,8,12 weeks after operation to monitor progression of IVD degeneration.Results The CT examinations showed there was significant difference in the degeneration signs at different time points between two groups （P ＜ 0.01）.The signals of nucleus pulposus were decreased in both two groups,and there was no significant difference between two groups.Pathologically,there was loss of nucleus pulposus and fibrosis of the IVDs in both two groups.Conclusion We built a practicable and reproducible animal IVD degeneration model via two possible surgical routes.The IVD degenerate fast at early time point via the far-lateral approach.

关 键 词：低温等离子消融 椎间盘退变 模型 动物 兔 

分 类 号：R681.53[医药卫生—骨科学]