‘灵武长枣’果实糖积累与蔗糖代谢相关酶的关系  

Relation between sugar accumulation and sucrose-metabolizing enzymes in fruit of ‘Lingwuchangzao'(Zizyphus jujuba Mill.)

作　　者：章英才[1] 陈亚萍[1] 景红霞[1] 苏伟东 

机构地区：[1]宁夏大学生命科学学院,银川750021 [2]宁夏红枣工程技术研究中心,宁夏灵武750400

出　　处：《果树学报》2014年第2期250-257,共8页Journal of Fruit Science

基　　金：国家自然科学基金(31160057)

摘　　要：【目的】研究‘灵武长枣’果实生长发育过程中糖的代谢积累与蔗糖代谢相关酶活性的关系。【方法】以‘灵武长枣’为试材,测定了果实的动态生长指标,以及果实中葡萄糖、果糖、蔗糖、淀粉的含量和蔗糖代谢相关酶的活性。【结果】(1)‘灵武长枣’果实生长曲线为双S型,早期果实主要积累葡萄糖和果糖,后期果实迅速积累蔗糖,果实成熟时主要积累蔗糖。(2)在果实的整个发育期,中性转化酶(NI)活性一直较低,酸性转化酶(AI)在前期活性很高,之后随果实发育其活性迅速降低直到果实成熟;蔗糖磷酸合成酶(SPS)的活性变化呈先高后低的下降趋势;蔗糖合成酶(SS)在果实发育前期分解方向酶活性(SSd)高于合成方向酶活性(SSs),果实发育中后期则主要以合成方向酶活性为主,分解方向活性随果实发育而下降,而合成方向活性则随果实发育而逐步增强。(3)果实发育前期,酸性转化酶(AI)、中性转化酶(NI)和蔗糖合成酶(SS)共同参与了蔗糖的分解代谢,果实发育后期,蔗糖合成酶(SS)合成方向酶和蔗糖磷酸合成酶(SPS)是催化蔗糖合成的重要酶。【结论】SSs在‘灵武长枣’果实的糖代谢中起主要的调控作用。[Objective]The aim of the paper was to study the relationship between the sugar accumulation and its metabolizing enzymes in development fruit. [Method] ＇Lingwuchangzao＇ （Zizyphus jujuba Mill.） was uses as materials to investigate the dynamic growth index of fruit, the content of glucose, fructose, su- crose , starch and the activities of sucrose metabolism-related enzymes. [Result] （ 1 ）The fruit growth was ＇double S＇ curve,and the fruit mainly accumulated glucose and fructose at the early stage,sucrose was accumulated rapidly in late period,and the mature fruit mainly accumulated sucrose. （2）The neutral in- vertase （NI） activity was low in the whole growth development period of fruit, the acid invertase （AI） activi- ty was high at the early stage but dropped rapidly with the fruit development until the fruit matured. The activity of sucrose phosphate synthase （SPS） was on the decrease which started high and ended low, the su- crose synthase （SS） activity in decomposition direction was higher than that in the synthetic direction at the early stage, the sucrose synthase （SS） activity in synthetic direction was chief in late period, and the su- crose synthase activity in the decomposition direction （SSd） reduced but increased gradually in the sucrose synthesis direction （SSs） with fruit development. （3）The AI , NI and SS partook together the catabolism of sucrose at the early stage of fruit development,while the sucrose synthase （SS） in the synthetic direction and sucrose phosphate synthase （SPS） are chief enzymes which catalyzed synthesizing sucrose in the fruit development late period.[Conclusion]The sucrose synthase （SS） in the synthetic direction plays the important regulative role in sugar metabolism in fruit of Zizyphus jujuba Mill. ＇Lingwuchangzao＇.

关 键 词：'灵武长枣’ 果实 糖积累 蔗糖代谢相关酶 

分 类 号：S665.1[农业科学—果树学]