金属硫蛋白对铅染毒小鼠的排铅作用研究  

Effect of metallothionein on lead excretion of lead exposed mice

作　　者：杨惠琴[1] 张睿[1] 丁世彬[1] 廖丹[1] 兰泽桥 李义勇 曹玉广[1] 应晨江[1] 左学志[3] 

机构地区：[1]华中科技大学同济医学院公共卫生学院,湖北武汉430030 [2]武汉天峡鲟都科技有限公司,湖北武汉430030 [3]华中科技大学附属同济医院,湖北武汉430030

出　　处：《工业卫生与职业病》2014年第2期105-107,113,共4页Industrial Health and Occupational Diseases

基　　金：国家自然科学基金资助项目(30972473)

摘　　要：目的探讨金属硫蛋白(metallothionein,MT)对染铅小鼠的排铅作用。方法选择雄性昆明种小鼠40只,随机分为正常对照组,醋酸铅模型组,MT组(MT低、高剂量组),依地酸二钠钙组,共5组,每组8只。除正常对照组外,其余4组小鼠连续30d自由饮用0.55g/L醋酸铅水溶液制作铅中毒模型。MT低、高剂量组小鼠在染铅同时以不同剂量MT灌胃干预;依地酸二钠钙组小鼠染铅同时每天肌肉注射依地酸二钠钙。结果 MT组小鼠血铅和股骨铅含量明显低于醋酸铅模型组,差异有统计学意义(P<0.05);MT组血清中的丙二醛(MDA)含量低于醋酸铅模型组,而总抗氧化能力(T-AOC)含量高于醋酸铅模型组,差异有统计学意义(P<0.05)。结论金属硫蛋白具有明显的排铅作用和一定的抗氧化作用。Objective To observe the effect of metallothionein（MT）on lead excretion of lead exposed mice. Methods 40 male Kunming mice were randomly divided into 5 groups with 8 mice in each group, i. e. normal control group, lead acetate model group, low dose MT group, high dose MT group and EDTA--Ca- Na2 group. With exception of the normal control, all mice of other four groups freely drank 0. 55g/L lead ac- etate solution for 30 days to establish lead poisoning model. Mice of both the low and high dosages MT groups were simultaneously instilled with MT when they were exposed to lead. A positive drug control group was set- up by simultaneous CaNa2 --EDTA injection. Results Lead levels in mice blood and femur of MT groups were much lower than those of the lead poisoning model group（P〈0. 05）. The MDA contents in mice blood of MT groups were lower than those of lead poisoning model group （P〈0. 05）. The contents of T--AOC in mice blood of MT groups were higher than those of lead poisoning model group （P〈0. 05）. Conclusions MT is effective in the process of lead excretion and shows a certain extent of anti--oxidative capability.

关 键 词：金属硫蛋白 排铅作用 抗氧化 小鼠 

分 类 号：R135[医药卫生—劳动卫生]