甘蓝型油菜(Brassica napus L.)RIL群体抗倒伏及其相关性状的QTL分析  

QTL mapping for lodging resistance and its related traits by RIL population of Brassica napus L.

作　　者：李扬[1,2] 顾慧[2] 戚存扣[2] 

机构地区：[1]南京农业大学农学院,江苏南京210095 [2]江苏省农业科学院经济作物研究所,江苏南京210014

出　　处：《中国油料作物学报》2014年第1期10-17,共8页Chinese Journal of Oil Crop Sciences

基　　金：农业部948重大项目(2011-G23);国家科技支撑计划(2010BAD01B10);国家油菜产业技术体系(CARS-13);江苏省农业科技自主创新资金[CX(11)4009]

摘　　要：油菜倒伏严重影响了油菜的产量和品质，为了定位甘蓝型油菜（Brassica napus L．）与抗倒伏性状相关的QTL，用抗倒伏品系浙平1号和易倒伏品系高芥1号组合重组自交系（RIL）家系为材料，通过两年两点试验，对该RIL群体的F2：6和F2：7，家系抗压力性状进行了QTL分析，并应用一年两点数据对该群体F2：6。家系结角层性状进行了QTL分析。结果表明，在4个试验中共检测到11个家系抗压力QTL，可解释4．41％～52．64％的表型变异，LOD值范围是2．68～26．58。其中qRP16-1可以在3个不同的试验中（南京-2012、溧水-2012和南京-2013）重复检测到，面P1、qRP4和qRP8-1同时在2个不同的试验中（溧水-2012和溧水-2013）重复检测到。qRP2-1、qRP2-2、qRP8-2、qRP8—3、qRPl6—2、qRP3和qRP8—4等7个QrL只能在特定的试验中检测到。同一地点不同年份间家系抗压力性状QTL相对同一年份不同地点间易被重复检测到。在2个试验中共检测到2个结角层厚度QTL，位于第15连锁群的qTPLl5在2个试验中重复检测到。Lodging causes yield loss and quality deficiency severely in oilseed rape. To mapping QTLs related to lodging resistance, 243 lines of a recombinant inbred line （RIL） population （ F2：6 and F2：7 ） of Brassica napus L. were tested at two locations in two consecutive years. The RIL population was derived from Zheping 1 （lodging resistant） x Gaojie 1 （lodging susceptible）. Results showed 11 QTLs for plant pressure resistance （PPR） using inclusive composite interval mapping from 4 trials. Their phenotype variation and LOD value ranges were 4.41% - 52.64% and 2.68 -26.58 respectively. Among QTLs, qRPI6 -1 was co -localized by 3 trials （Nanjing- 2012, Lishui - 2012 and Lishui - 2013 ）. qRP1, qRP4 and qRP8 - 1 were co - localized by 2 trials （ Lishui - 2012 and Lishui-2013）. And qRP2- 1, qRP2-2, qRP8-2, qRP8-3, qRP16-2, qRP3 and qRP8-4 were only local- ized by one trial. QTLs for PPR were co -localized more easily at single location in each year than those in single year at each location. Two QTLs for thickness of pod - set layer were identified from 2 trials, qTPL15 which on LG15 was eo -localized by 2 trials.

关 键 词：甘蓝型油菜 重组近交系 抗倒伏 抗压力 结角层 QTLS 

分 类 号：S565.403[农业科学—作物学] S332.7