ISSR标记分析油菜黑胫病原菌遗传多样性  

Genetic diversity of phoma stem canker pathogen Leptosphaeria biglobosa by ISSR

作　　者：郝丽芬[1,2] 宋培玲[1,2] 李子钦[1] 皇甫海燕[1] 李强生[3] 

机构地区：[1]内蒙古农牧业科学院植物保护研究所,内蒙古呼和浩特010031 [2]内蒙古大学生命科学院,内蒙古呼和浩特010021 [3]安徽省农业科学院作物研究所,安徽合肥230031

出　　处：《中国油料作物学报》2014年第1期98-105,共8页Chinese Journal of Oil Crop Sciences

基　　金：国家科技支撑项目(2010BAD01B04);内蒙古创新基金(NMGCX201203);内蒙古科技创新平台建设(NMGKJHZ2012001)

摘　　要：利用ISSR分子标记技术分析了油菜黑胫病菌（Leptosphaeria biglobosa）的遗传多样性。分离获得国内菌株84株，并以15株国外（英国、加拿大、波兰）油菜黑胫病菌菌株作为参照，利用筛选出的24条引物对这99株病原菌进行ISSR—PCR扩增。共扩增出245条带，遗传相似性系数为0．65～1．00，国内和国外油菜黑胫病菌菌株很明显被分为两个菌群，中国84株菌基本按地理来源分为5个类群，只有江苏省菌株分布于各个类群。各省的Nei’s基因多样性指数范围是0．1081～0．2527，而香农（Shannon’S）信息指数的范围是0．1575～0．3726，顺序为江苏〉湖北〉安徽〉内蒙古〉上海〉四川。中国与英国和加拿大菌株的遗传一致度分别为0．8343和0．7839。The genetic diversity of Leptosphaeria biglobosa, causing phoma stem canker on oilseed rape, was analyzed using ISSR （inter simple sequence repeats） marker. 8d blackleg isolates were collected from China and 15 from the United Kingdom, Canada and Poland were used as control. A total of 245 bands generated by the ISSR - PCR with 24 selected primers were polymorphic. The dendrogram constructed with 99 individuals using UPGMA （unweighted pair group mean analysis ） showed that Chinese isolates were different from Canada and Europe. Meanwhile, Chinese isolates from 7 provinces were almost divided into 5 groups according to their geographical locations only isolates from the Jiangsu Province distributed into other groups. Nei＇ s gene diversity ranged from 0. 252 7 （in Jiangsu） to 0. 108 1 （in Sichuan）, with the order of Jiangsu 〉 Hubei 〉 Anhui 〉 Inner Mongolia 〉 Shanghai 〉 Sichuan. Shannon＇ s information index were from 0. 157 5 to 0. 372 6. The Nei＇ s gene identity were 0. 783 9 between the China and Canada isolates, and 0. 834 3 between China and the United Kingdom isolates.

关 键 词：油菜黑胫病 LEPTOSPHAERIA biglobosa ISSR 遗传多样性 地理来源 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]