紫外可见分光光度法测定叠鞘石斛中联苄类化合物的含量  

Determination of Bibenzyl Compounds Content in Dendrobinm denneanum by UV-Visible Spectrophotometry

作　　者：贾芳[1] 夏厚林[1] 黄萍[1] 黄小燕[1] 宁梓君[1] 唐建军[1] 张廷模[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137

出　　处：《成都中医药大学学报》2014年第1期7-10,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：科技部"十二五"支撑计划项目(编号:2011BAI13BO2-8)

摘　　要：目的：建立叠鞘石斛中联苄类化合物的含量测定方法。方法：以石斛酚为对照品，未加显色剂条件下，建立紫外可见分光光度法测定叠鞘石斛中联苄类化合物的含量。结果：确定最佳吸收波长280nm，石斛酚在浓度4．88-58．56μLg／mL范围内呈良好线性关系（r=0．9998），平均加样回收率为97．39％，RSD=2．97％。结论：该方法准确、简单、快捷，重现性好，适用于叠鞘石斛中联苄类化合物含量的测定。Objective： To establish the content determination method for bibenzyl compounds in Dendrobinm denneanum. Methods： Under the condition of not adding chromogenic agent, using ultraviolet and visible spectmphotometfic determi- nation to detect the content of bibenzyl compounds with gigantol as the standard substance. Results： The best absorption wavelength was 280 nm. Gigantol had a liner relationship with the absorbance in the range of 4. 88 - 58. 56 p.g/mL （ r = 0. 9998 ） . The average re- covery for gigantol was 97. 39%, RSD was 2.97%. Conclusion： The method is simple, sensitive, accurate and can be used for bibenzyl compounds determination in Dendrobinm denneanum.

关 键 词：叠鞘石斛 石斛酚 联苄类化合物 含量测定 紫外可见分光光度法 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]