藏药大车前草中大车前苷的定性和定量分析  

Qualitative and Quantitative Analysis of Plantamajoside in Plantago major L.

作　　者：吴恋[1] 王春艳[1] 吕维[1] 叶娜[1] 罗红丽[1] 江道峰[1] 范成杰[1] 万丽[1] 

机构地区：[1]成都中医药大学药学院,教育部中药材标准化重点实验室,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《成都中医药大学学报》2014年第1期48-50,59,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：四川省食品药品监督管理局<四川省藏药材标准>(2013版)标准研究课题

摘　　要：目的：建立藏药大车前草药材的定性定量方法。方法：以大车前苷为指标性成分，以硅胶G为吸附剂，乙酸乙酯-甲醇-甲酸-水（18：3：1．5：1）为展开剂，紫外光灯（365nm）下检测，建立大车前草药材的薄层色谱鉴别方法。用高效液相色谱法进行含量测定，DIKMADiamonsilC18 色谱柱（250x4．6mm5μm）；流动相为乙腈-0．1％磷酸（18：82），流速：1mL／min；检测波长330nm；柱温：30℃。结果：大车前草药材薄层色谱在紫外光灯（365nm）下检识，大车前苷呈亮蓝色荧光斑点，斑点清晰，分离效果佳；高效液相色谱法含量测定大车前苷在0．09-11．22μg呈线性（r=0．999）；加样回收率为100．64％（RSD=0．95％）；测得4批大车前草中大车前苷的含量在0．094％-1．361％。结论：通过对4批大车前草中的大车前苷进行定性和定量分析，证明所建立的薄层色谱鉴别和高效液相色谱法含量测定方法简便可靠，准确度高，重复性好，为大车前草的质量标准建立提供了实验依据。Objective： To establish qualitative and quantitative methods to analysis the Plantago major L. Methods： Using plantamajoside as the maker compound. A TLC method was developed to identify the plantamajoside in 4 Plantago major L. sample. Using silica gel G as coating substance and a mixture of ethyl acetate- methanol- formic acid-water （ 18： 3： 1.5：1 ） as a de- veloping solvent, examining under the ultraviolet lamp （365 nm）; A HPLC method was established to determine the content of plan- tamajoside in the samples. Plantamajoside was separated at 30°C on a DIKMA Diamonsil C18 （250 x 4. 6 mm 5 μm） column with ace- tonitrile -0. l%phosphorie acid （18： 82） as the mobile phase. The detection wavelength was set at 330 nm and the flow rate was 1 mL/min. Results： The tested samples and the marker compound plantamajoside showed as a distinct light-blue fluorescence spot ob- served under UV 365 nm, the separation effect was excellent. In the HPLC determination, good linearity of the calibration curve be- tween the peak area and the concentration of plantamajoside was obtained in the range of 0. 09 - 11.22 μg （ r = 0. 999）, and the aver- age recovery of plantamajoside was 100. 64% with a RSD of 0. 95%. The contents of plantamajoside were in the range of 0. 0935% - 1.31% in the samples. Conclusions： The established TLC identification and HPLC determination were sensitive, reliable and repeat- able, which can be applied for the quality evaluation and standard criteria of Plantago major L.

关 键 词：大车前草 大车前苷 薄层色谱 高效液相色谱 质量标准 

分 类 号：R291.408[医药卫生—民族医学] R284.1[医药卫生—中医学]