BacT/ALERT 3D检测技术应用于结核分枝杆菌快速培养的效果评价  

The effect evaluation on the application of BacT /ALERT 3D technique for the fast cultivation of mycobacterium tuberculosis

作　　者：黄文忠[1] 王平平[1] 周燕珍 吴红照[1] 胡真宝[1] 

机构地区：[1]浦江县疾病预防控制中心,浙江浦江322200

出　　处：《浙江预防医学》2014年第3期217-220,共4页Zhejiang Journal of Preventive Medicine

基　　金：金华市科学技术研究项目(2010-3-082)

摘　　要：目的评价BacT/ALERT 3D快速培养系统对结核分枝杆菌(MTB)的检测和肺结核早期诊断的应用价值。方法采用BacT/ALERT 3D液体培养技术和L-J固体培养法分别对病例标本培养,比较2种方法检出结果及检出时间。结果平均出现阳性天数BacT/ALERT 3D法为(13±2.05)d,L-J固体培养法为(34.7±4.76)d,差异有统计学意义(P<0.05);BacT/ALERT 3D法和L-J固体培养法对治疗2月后肺结核病例的涂阳标本MTB培养阳性检出率分别为71.18%(42/59)和67.80%(40/59),而对涂阴标本BacT/ALERT 3D法MTB培养阳性率39.62%(42/106)高于L-J固体培养法阳性率26.42%(28/106),差异有统计学意义(P<0.05)。结论与传统L-J固体培养法比较,BacT/ALERT 3D液体培养技术明显缩短检出时间,并能提高低浓度(痰涂阴)标本的结核分枝杆菌阳性检出率。Objective To evaluate the BacT/ALERT 3D liquid rapid culture system for the rapid detection of mycobacterium tuberculosis（MTB）and early diagnosis of pulmonary tuberculosis.Methods BacT/ALERT 3D liquid culture mediums and lowenstein-jensen（L-J）solid culture mediums were applied to detect Mycobacterium tuberculosis sputum specimens respectively.Analysis of detection results and detection time were also performed.Results Average positive days of BacT/ALERT 3D liquid culture medium was（13 ±2.05）days,which was shorter than that of L-J solid culture mediums（34.7 ±4.76 ）d （P〈0.05 ）.Compared to the L -J solid culture mediums,BacT/ALERT 3D liquid culture medium had higher positive rate for 59 patients whose sputum smear test was positive,and the positive rate were 71.18%（42/59）and 67.80%（40/59）respectively（P〈0.05）.BacT/ALERT 3D liquid culture medium had higher positive rate than L-J solid culture mediums for 106 patients whose sputum smear test was negative,and the positive rate were 39.62%（42/106）and 26.42%（28/106）respectively（P〈0.05）.Conclusion Compared to the traditional L-J solid culture system,BacT/ALERT 3D liquid culture system can shorten detection time and improve the positive detection rate of MTB in specimens with low concentration.

关 键 词：结核分枝杆菌 L-J固体培养法 效果评价 

分 类 号：R378.911[医药卫生—病原生物学]