常用大鼠血瘀证模型的比较研究  

Study on commonly used rat model of blood stasis syndrome

作　　者：宋程程[1] 王志斌[2] 苏斌[1] 郭玉东[2] 高阳[2] 左泽平[2] 金家金 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京市药检所

出　　处：《北京中医药大学学报》2014年第2期94-98,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家药典委员会课题活血化瘀类中药的生物活性测定方法研究(No.1180)

摘　　要：目的采用6种造模方法制备大鼠血瘀模型,比较不同大鼠血瘀模型的血液流变学指标及凝血参数的差异性,探讨各个模型的特点及适用性。方法分别制备寒凝血瘀、肾上腺素、右旋糖酐、高脂寒凝、高脂血症及血虚血瘀6种大鼠血瘀模型,比较不同模型全血黏度、血浆黏度、红细胞聚集变形性、红细胞压积(HCT)、血小板聚集性、凝血酶原时间(PT)、活化部分凝血酶时间(APTT)和纤维蛋白原(FIB)含量的差异性。结果肾上腺素、高脂血症、高脂寒凝及血虚血瘀大鼠血瘀模型均能改变血液流变学指标及凝血参数,其中肾上腺素血瘀模型影响较为显著,适用性较好;右旋糖酐血瘀模型能显著影响血液流变学指标,特别是红细胞相关指标较为显著;寒凝血瘀模型能显著影响凝血参数。结论不同造模方法建立的大鼠血瘀模型在血液流变学指标及凝血参数上的影响不同,为在活血化瘀药物活性的研究中模型的精确选择提供了参考。Objective To compare the hemorheology indexes and coagulation parameters of 6 rat models of blood stasis syndrome which were established by using six different methods, and to discuss the characteristics and applicability of each rat model. Methods Six different rat models of blood stasis syndrome were established including congealing cold with blood stasis group, adrenaline group, dextranum group, hyperlipidemia with congealing cold group, hyperlipidemia group and blood deficiency with blood stasis group. The differences in whole blood viscosity （WBV） , plasma viscosity, erythrocyte deformability, hematocrit （HCT）, platelet aggregation, prothrombin time （PT）, activated partial thromboplastin time （AP＇IT） and fibrinogen （FIB） content were compared among all groups. Results The hemorheology indexes and coagulation parameters were improved in adrenaline group, hyperlipidemia group, hyperlipidemia group and blood deficiency with blood stasis group, which was more significant in adrenaline group with higher applicability. The hemorheology indexes were significantly influenced in dextranum group, especially RBC-related parameters. The coagulation parameters were significantly influenced in congealing cold with blood stasis group. Conclusion The rat models of blood stasis syndrome established by using different methods show different characteristics in hemorheology indexes and coagulation parameters, and have different applicability. This study provides some reference forchoosing rat models of blood stasis syndrome in the research on the activities of medicinals with the actions of activating blood and resolving stasis.

关 键 词：血瘀证模型 血液流变学 抗凝血 大鼠 

分 类 号：R285.5[医药卫生—中药学]