益气活血方和补肾生髓方对局灶性脑缺血再灌注大鼠Ctnnb1和Krt1基因及其蛋白表达的影响  

Influences of Yiqi Huoxue Fang and Bushen Shengsui Fang on genetic and protein expressions of Ctnnb1 and Krt1 in rats with focal cerebral ischemia /reperfusion

作　　者：徐伟[1] 胡建鹏[1] 王键[1] 吴生兵[1] 王丽娜[1] 何玲[1] 菅威[1] 谭辉[1] 

机构地区：[1]安徽中医药大学省部共建新安医学教育部重点实验室,安徽230038

出　　处：《北京中医药大学学报》2014年第2期112-115,I0004,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家科技支撑计划项目(No.2012BAI26B01);国家自然科学基金项目资助(No.30973692)

摘　　要：目的探讨益气活血方和补肾生髓方对脑缺血再灌注大鼠Ctnnb1和Krt1基因及其蛋白表达的影响。方法制作局灶性脑缺血再灌注大鼠模型,随机分为假手术组、模型组、益气活血方组和补肾生髓方组,脑缺血2 h后再灌注。采用PCR法检测缺血侧额顶叶皮质Ctnnb1和Krt1基因表达,用免疫组化Envision两步法检测缺血侧额顶叶皮质区和海马CA1区蛋白表达。结果与假手术组比较,模型组Ctnnb1和Krt1基因表达显著上调(P<0.05);与模型组比较,益气活血方组Ctnnb1和Krt1基因表达显著下调(P<0.05),补肾生髓方组Ctnnb1基因表达显著下调(P<0.05),益气活血方组Ctnnb1基因下调与补肾生髓方组有显著差别(P<0.05)。在额顶叶皮质区和海马CA1区,与假手术组比较,模型组Ctnnb1和Krt1蛋白表达显著升高(P<0.05);与模型组比较,益气活血方组与补肾生髓方组Ctnnb1和Krt1蛋白表达均显著降低(P<0.05);益气活血方组Ctnnb1蛋白表达显著低于补肾生髓方组(P<0.05)。结论两种中药复方能通过降低皮质或海马Ctnnb1和Krt1基因及其蛋白表达,调节Notch信号通路,促进局灶性脑缺血再灌注损伤脑组织修复。Objective To investigate the influences of Yiqi Huoxue Fang and Bushen Shengsui Fang on the genetic and protein expressions of Ctnnbl and Krtl in rats with focal cerebral isehemia/reperfusion. Methods The rat model of focal cerebral ischemia/reperfusion was established. The rats were randomly divided into sham group, model group, Yiqi Huoxue Fang group （Y group） and Bushen Shengsui Fang group （B group）. After cerebral ischemia for 2 h and reperfusion, the genetic expressions of Ctnnbl and Krtl in ischemic frontoparietal cortex were detected by using PCR assay, and protein expressions of Ctnnbl and Krtl in ischemie frontoparietal cortex and hippocampus CA1 zone were detected by using immunohistochemistry Envision two - step method. Results The genetic expressions of Ctnnbl and Krtl increased significantly in model group compared with sham group （P 〈 0. 05 ）, and decreasedsignificantly in Y group compared with model group （ P 〈 0.05 ）. The genetic expression of Ctnnbl decreased significantly in B group （P 〈 0.05 ）, which had significant difference compared with Y group （P 〈 0.05 ）. The protein expressions of Ctnnbl and Krtl in frontoparietal cortex and hippocampus CA1 zone increased significantly in model group compared with sham group （ P 〈 0.05 ） , and decreased significantly in Y group compared with model group （P 〈 0.05 ）. The protein expression of Ctnnbl was significantly lower in Y group than that in B group （ P 〈 0.05 ）. Conclusion Yiqi Huoxue Fang and Bushen Shengsui Fang can improve the repairing of injured cerebral tissue through reducing the the genetic and protein expressions of Ctnnbl and Krtl in cortex and hippocampus and regulating notch signal pathway.

关 键 词：益气活血方 补肾生髓方 脑缺血再灌注 海马CA1区 Ctnnb1 Krt1 大鼠 

分 类 号：R285.5[医药卫生—中药学]