水煎煮法提取骨碎补总黄酮的动力学模型适应性研究  

Kinetic model applicability of total flavonoids extracted from Gusuibu( Rhizoma Drynariae) by using water decoction method

作　　者：张晋[1] 李新存[2] 葛亮[1,3] 祁娟娟[1] 曹飒丽[1] 张慧[1] 党晓芳[1] 杨博迪 倪健[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]郑州大学第一附属医院药学部 [3]新疆医科大学中药学院

出　　处：《北京中医药大学学报》2014年第2期121-125,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目资助(No.81173563);北京中医药大学复方中药制药创新团队(No.2011-CXTD-13)

摘　　要：目的以骨碎补中的总黄酮为测定指标,研究水煎煮过程中的提取动力学机制并拟合出提取动力学方程。方法首先对试验室已有的提取动力学模型进行简化推导,然后依据模型的参数及特点,用紫外分光光度法测定不同提取时间和溶剂下的提取液浓度,以Fick扩散定律为基础,推算出方程参数,将测定结果代入数学模型进行方程拟合。结果骨碎补提取液中总黄酮质量浓度的自然对数值随时间、溶剂倍量的自然对数值变化呈良好的线性关系,根据不同条件下线性回归方程的斜率和截距,计算出模型中参数n=-0.323 54,a=27.730 05,f1=0.023 019并建立了方程,验证结果准确,标准偏差均控制在工业可接受范围内。结论所建立的模型适用并能良好地描述骨碎补水提取的动力学过程。Objective To study the extraction kinetic mechanism and fit out extraction kinetics equation during water decocting taken total flavonoids extracted from Gusuibu （Rhizoma Drynariae ） as determination index. Methods At first, the existing model in laboratory was simplified and deduced, and then the concentration of extracting solution with different extraction time （t） and solvent loading （M） was detected by using ultraviolet spectrophotometry according to the parameters and characteristics of the model. The parameters of equation were deduced based on Fick＇ s scattering laws, and the results were substituted into mathematic model for equation fitting. Results The natural logarithm of concentration of total flavonoids had good linear relationship as t and M were redoubled. According to the slopes and intercepts of linear regression equation under different conditions, the calculated results as follows： n = -0. 323 54, a =27.730 05 and fl =0. 023 019. A equation was established with accurate detecting results and standard deviation was controlled within industrial tolerance interval. Conclusion The established model can be applied to and descript well the kinetic extraction course of Gusuibu （ Rhizoma Drynariae）.

关 键 词：骨碎补 提取动力学模型 模型简化 总黄酮 

分 类 号：R283.1[医药卫生—中药学]