重组水泡性口炎病毒基质蛋白制备及ELISA检测方法的建立  

Preparation of Recombinant VSV Matrix Protein and Establishment of ELISA for Antibody Detection

作　　者：张世宽[1] 陈备娟 肖贤 孙涛[4] 毛慧玲[1] 

机构地区：[1]南昌大学生命科学与食品工程学院,南昌330047 [2]宝山区动物疫病预防控制中心,上海201900 [3]上海市种猪场,上海201408 [4]上海市兽医生物技术重点实验室,上海交通大学农业生物学院,上海200240

出　　处：《上海交通大学学报（农业科学版）》2014年第1期89-94,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家自然科学基金(31272562)

摘　　要：水泡性口炎由水泡性口炎病毒(VSV)引起。VSV基因组编码5个结构蛋白,其中基质蛋白(matrix protein,M)是其重要毒力因子,可阻遏宿主细胞mRNA由胞核向细胞质的转运,并抑制感染病毒的细胞产生I型干扰素IFNα/β,使病毒得以快速繁殖。本实验克隆了印第安那型VSV的M基因,在大肠杆菌表达系统中表达制备重组M蛋白,以M蛋白为抗原建立了检测特异M蛋白抗体的间接ELISA方法。采用建立的ELISA方法分析了VSV感染小鼠体内M蛋白抗体的变化规律。The pathogen of VS is vesicular stomatitis virus （VSV）. VSV genome consists of five structural protein genes. Matrix protein （M） is an important virulence factor for VSV,which can inhibit host mRNA transport from nucleus outside to cytoplasm in host cells. As the result, production of type I interferon by host cells can be inhibited,whereas viruses can replicate efficiently in infected cell. In our study,M gene was cloned and expressed in E. coli. Based on the recombinant M protein, indirect ELISA assay was established to detect antibody against M in VSV-inoculated SPF mice.

关 键 词：水泡性口炎病毒 基质蛋白 ELISA 

分 类 号：S855.3[农业科学—临床兽医学]