薄皮甜瓜离体再生体系的优化  

Optimization of Plant Regeneration System in Oriental Melon

作　　者：付秋实[1] 曹芸运 谭明明[3] 王烨[1] 郭仰东[2] 王怀松[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]中国农业大学农学与生物技术学院,北京100193 [3]四川农业大学园艺学院,四川雅安625014

出　　处：《中国瓜菜》2014年第2期16-19,共4页China Cucurbits And Vegetables

基　　金：国家自然科学基金(No.31201642);现代农业产业技术体系建设专项(CARS-26-07)

摘　　要：通过器官发生途径诱导形成不定芽,建立薄皮甜瓜‘IVF05’植株再生体系,探讨不同外植体及不同的激素组合对不定芽再生的影响.结果表明,子叶近胚轴端外植体的不定芽再生率为90.00％,子叶节的再生率为85.00％,子叶远胚轴端外植体的再生率为31.43％,下胚轴的再生率为0,子叶近胚轴端是‘IVF05’不定芽分化的理想的外植体.不定芽诱导中最适宜的培养基为MS＋ ABA 0.5 mg· L-1＋ 6-BA 1.0 mg· L-1,在此培养基上产生的愈伤较少,能正常分化的不定芽较多,不定芽的生长较快.在MS＋ 6-BA0.05 mg· L-1的不定芽伸长培养基上,分化的不定芽能够伸长长大.在MS＋ IAA 0.2 mg·L-1的生根培养基上无根苗容易生根.从外植体培养到获得完整再生植株需50～60d.An efficient regeneration system of planttets was established for melon （Cucumis melo L. ＇ IVF05＇ ）. We investigated the influence of explant type and plant growth regulator combinations on regeneration rate of adventitious buds in this research. The results showed that the adventitious buds regeneration rates of cotyledon explants near the hypocoty] ,cotyledonary nodes, cotyledon explants far from hypocotyl and hypocotyl were 90.00% ,85.00% ,31.43% and 0 ,respectively. Cotyledon explants near the hypocotyls were the optimal explants. The optimal concentrations of plant growth regulators were 0.5 mg. L^-1 ABA plus 1.0 mg. L^-1 6-BA. The buds elongation medium was MS ＋ 6-BA 0.05 mg. L^-1. The rooting medium was MS ＋ IAA 0.2 mg. L^-1. It took about 50 to 60 days from explants culture to obtain complete regeneration plant.

关 键 词：薄皮甜瓜 离体再生 子叶 6-BA ABA 

分 类 号：S652[农业科学—果树学]