毛白杨翻译起始因子基因PtoeIF5A2的克隆及其表达特性分析  

Isolation and Expression of Eukaryotic Translation Initiation Factor 5A2 in Populus tomentosa

作　　者：管阳[1] 王宏芝[1] 张杰伟[1] 陈亚娟[1] 朱丹[1] 丁莉萍[1] 魏建华[1] 

机构地区：[1]北京市农林科学院北京农业生物技术研究中心 农业基因资源与生物技术北京市重点实验室,北京100097

出　　处：《林业科学》2014年第2期63-69,共7页Scientia Silvae Sinicae

基　　金：国家重点基础研究发展计划(973)项目(2012CB114501);国家高技术研究发展计划(863)项目(2011AA100201);国家自然科学基金项目(30972392)

摘　　要：真核翻译起始因子5A(eIF5A)在植物生长发育和抗逆过程中发挥着重要作用。以2个月的毛白杨‘BJHR01’幼苗总RNA反转录的cDNA为模板,克隆得到PtoeIF5A2(GenBank登录号:HQ529480)基因全长cDNA序列。该基因包含483 bp开放读码框,编码160个氨基酸,预测蛋白分子质量为18 kDa,等电点为5.76。Real-Time qRT-PCR分析表明:PtoeIF5A2在6个月毛白杨幼苗叶片中的表达量最高,约为其在幼根中表达量的5倍。PtoeIF5A2响应了干旱、ABA(200μmol·L-1)、低温(4℃),尤其是NaCl(300 mmol·L-1)胁迫。在NaCl胁迫24 h后,PtoeIF5A2的表达量上调25倍,结合其启动子中含有6个病原体与高盐响应(GT-1 box)元件,推测该基因在响应高盐胁迫时起着重要作用。Eukaryotic translahon initaatlon tactor 3A （elIY3A） plays an important role m plant development and various stresses. In this study, the full length PtoelF5A2 （GenBank Accession number： HQ529480） eDNA was isolated from the eDNA library prepared from 2-month-old Populus tomentosa ＇BJHR01＇ seedlings using RT-PCR technique. The PtoelF5A2 coding region contained 483 nucleotides, encoding 160 amino acids with the molecular weight of about 18 kDa and PI of 5.76. The real time quantitative RT-PCR displayed that the expression of PtoelF5A2 was highest in leaves in 6-month-old seedlings, about 5 times higher than that of in root. Moreover, the expression of PtoelF5A2 in P. tomentosa responded to the drought, ABA and low temperature, especially NaC1 treatment. After 24 h under NaC1, the PtoelF5A2 expression was 25 times higher than control, and the PtoelFSA2 promoter contained 6 response elements to pathogen and salinity, indicating that PtoelF5A2 would be mainly involved in response of salt stress.

关 键 词：真核翻译起始因子5A 毛白杨 盐胁迫 PtoeIF5A2 

分 类 号：S718.46[农业科学—林学]