HPLC法测定茯苓皮中松苓新酸  

Determination of dehydrotrametenolic acid in peels of Poria cocos by HPLC

作　　者：闫雪生[1] 赵许杰[2] 韩媛媛[2] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东中医药大学,山东济南250355

出　　处：《现代药物与临床》2014年第2期166-168,共3页Drugs & Clinic

摘　　要：目的建立茯苓皮中松苓新酸的HPLC测定方法。方法采用高效液相色谱法,Lichrospher C18色谱柱（200 mm×4.6mm,5μm）,流动相为乙腈–0.5%磷酸水溶液（80∶20）,检测波长为242 nm,体积流量为1.0 mL/min,柱温为30℃,进样量为10μL。结果松苓新酸在0.142 5~2.280 0μg与峰面积呈良好的线性关系（r=0.999 3）,平均回收率为101.83%,RSD值为1.45%（n=6）。结论该方法操作简便,稳定可行,重复性好,可用于茯苓皮中松苓新酸的测定。Objective To establish an HPLC method for the determination of dehydrotrametenolic acid in the peels of Poria cocos. Methods HPLC was carried out on a Lichrospher C18 column （200 mm×4.6 mm, 5 μm） with acetonitrile-0.5% phosphoric acid （80 ： 20） as mobile phase. The detection wavelength was set at 242 nm. The injection volume was 10 gL at the flow rate of 1.0 mL/min. The column temperature was at 30 ℃. Results The calibration curve was linear over the range of 0.142 5--2.280 0μg （r=0.999 3） for dehydrotrametenoli6 acid. The average recovery rate was 101.83% with RSD value at 1.45% （n=6）. Conclusion The established method is rapid, sensitive＇, and accurate, and could be used for the determination of dehydrotrametenolic acid in the peels of e. cocos.

关 键 词：茯苓皮 三萜类 松苓新酸 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]