火木层孔菌桑黄醇提物的HPLC指纹图谱研究  

Study on HPLC Fingerprint of Ethanol Extract of Phellinus igniarius

作　　者：俞忠明[1,2] 董宇[1,2] 李洪玉[1,3] 陈莉君[2] 寿旦[1,3] 

机构地区：[1]浙江省中医药研究院中药研究中心,浙江杭州310007 [2]浙江中医药大学,浙江杭州310053 [3]浙江省中西医结合肿瘤诊治重点实验室,浙江杭州310007

出　　处：《中药材》2014年第1期38-41,共4页Journal of Chinese Medicinal Materials

基　　金：中央财政补助地方条件专项;浙江省科技厅公益性技术应用研究(2012C33125);浙江省科技厅科技公共服务项目(2012F30027);浙江省中医药科技计划(2013ZQ002)

摘　　要：目的：建立10批不同产地火木层孔菌桑黄醇提物（EEPI）的HPLC指纹图谱,并对其质量进行评价。方法：采用SHISEIDO CAPCELL PAK C18色谱柱（250 mm×4.6 mm,5μm）,以0.2%磷酸水溶液-甲醇进行梯度洗脱,流速1.0 mL/min,检测波长为395 nm。以Inoscavin A为参比峰建立EEPI特征吸收峰共有模式,并通过峰重叠率计算、相似度评价系统、聚类分析等对其相似度进行评价。结果：在选定的色谱条件下,得到10批不同产地火木层孔菌桑黄指纹图谱,标定了9个共有峰,并建立了火木层孔菌桑黄的指纹图谱共有模式,相似度在0.652~0.995之间,聚类分析结果将10批火木层孔菌桑黄分为3个大类。结论：此方法简单、准确、重复性好,峰分离度较好,为有效地评价火木层孔菌桑黄的综合质量提供了参考依据。Objective：To establish the HPLC fingerprint of ethanol extract of Phellinus igniarius（ EEPI）,and to evaluate its quality.Methods：Adopted 0.2% phosphoric acid-methanol as mobile phase with a SHISEIDO CAPCELL PAK C 18 column（ 250 mm × 4.6 mm,5 μm）,the flow rate was 1.0 mL/min,and the detection wavelength was set at 395 nm.Inoscavin A was used as reference peak to establish common mode of characteristic absorption peaks of EEPI,and its similarity was evaluated by peaks overlap rate calculation,similarity evaluation system and cluster analysis.Results：Under the chromatographic conditions,HPLC fingerprint of EEPI was established,and nine common peaks were selected.The similarities was between 0.652 and 0.995.According to cluster analysis,10 batches of Phellinus igniarius were divided into three groups.Conclusion：This method is simple,accurate and repeatable with good separation.It can provide basis for the comprehensive quality evaluation of Phellinus igniarius.

关 键 词：火木层孔菌桑黄 HPLC指纹图谱 质量评价 相似度评价 

分 类 号：R282.5[医药卫生—中药学]