益气活血法对高糖损伤NRK-52E细胞摄取蛋白质的影响  

Effect of Supplementing Qi and Activating Blood Circulation Method on Protein Uptake in NRK-52E Cells Injured by High Glucose

作　　者：尹登科[1] 尹娟娟[1] 杨晔[1] 李云[1] 

机构地区：[1]安徽中医药大学药学院/现代中药安徽省工程技术研究中心,安徽合肥230031

出　　处：《中药材》2014年第1期91-94,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81102682);安徽省高等学校优秀人才基金(2011SQRL091)

摘　　要：目的:以黄芪丹参药对为代表考察益气活血法对高糖损伤肾小管上皮细胞(NRK-52E)摄取蛋白质功能的影响。方法:MTT法考察黄芪丹参合用对高糖损伤NRK-52E细胞增殖的影响,荧光显微镜及荧光分光光度计分析NRK-52E对FITC-BSA的摄取作用,免疫组化观察megalin表达。结果:益气活血法能剂量依赖性地减少高糖引起NRK-52E细胞数减少;高糖能导致NRK-52E细胞对FITC-BSA摄取减少,megalin表达减少,益气活血法能改善NRK-52E细胞对FITC-BSA的摄取,增加megalin表达。结论:益气活血法能保护高糖损伤NRK-52E细胞,并且通过增加megalin表达,减少高糖对NRK-52E摄取蛋白质功能的损伤。Objective：To study the effect of supplementing Qi and activating blood circulation method with danshen and huangqi on protein uptake in NRK-52E cells injured by high glucose.Methods：The influence of combined use of huangqi and danshen on NRK-52E cells proliferation were determined by MTT method,FITC-BSA uptake were observed by fluorescence microscope and analyzed by fluorescence spectrophotometer,and megalin expression were detected by the method of immunohistochemistry.Results：Combined use of huangqi and danshen could strengthen its protection effect.The FITC-BSA uptake and expression of megalin in NRK-52E cells were decreased in high glucose environment.Combined use of huangqi and danshen could increase FITC-BSA uptake and megalin expression in high glucose-injured NRK-52E cells.Conclusion：The method of supplementing Qi and activating blood circulation can protect high glucose injured NRK-52E cells and improve the function of protein uptake by increasing expression of megalin.

关 键 词：益气活血法 高糖 NRK-52E MEGALIN 

分 类 号：R285.5[医药卫生—中药学]