KGF及其联合G-CSF对^(137)Cs γ射线5.0Gy照射小鼠重要脏器的放射防护作用  

Radioprotective Effects of KGF and Its Combination with G- CSF on Important Organ in ^(137)Cs γ- ray 5Gy Irradiated Mice

作　　者：张鹏程[1] 曹永珍[1] 于程程[2] 姜恩海[2] 

机构地区：[1]天津医科大学总医院肿瘤放射治疗科,天津300052 [2]中国医学科学院北京协和医学院放射医学研究所

出　　处：《中国辐射卫生》2014年第1期7-11,共5页Chinese Journal of Radiological Health

基　　金：卫生行业科研专项资助项目(201002009)

摘　　要：目的研究角质细胞生长因子(KGF)联合粒细胞集落刺激因子(G-CSF)对受照小鼠造血及重要脏器的放射防护作用。方法将6至8周龄C57BL/6小鼠共70只随机分成5组:空白对照组、单纯照射组、KGF给药组、G-CSF给药组、KGF联合G-CSF给药组,经137Cs-γ射线一次性全身照射后分别给药,通过体重、脏器指数、各脏器SOD活性、MDA含量和外周血(WBC、RBC、Hb、PLT)等指标分析KGF及G-CSF对其血液和重要脏器的影响。结果受照后,KGF组与G-CSF组小鼠体重回升较明显;与G-CSF组相比,联合组肺指数差异有统计学意义(P<0.05);外周血RBC、Hb、PLT的含量指标中,联合组高于其余各给药组;G-CSF组肝、心的SOD活性高于KGF组和联合组;KGF组的肺SOD活性高于G-CSF组和联合组。结论本研究表明联合组对小鼠造血系统的修复优于其他给药组;GCSF对于肝脏和心脏的抗氧化能力更有效;KGF对于肺部的抗氧化能力更有效。Objective To study the protective effects of keratinocyte growth factor （KGF） and granulocyte colonystimulating factor （ G - CSF） on hematopoietic system and important organs in irradiated mice. Methods 70 6 - to 8 - week old C57BL/6 mice were chosen as the objects on of study. They were randomly divided into 5 groups： the control group, irradiation group, KGF group, G - CSF group and the KGF plus G - CSF group, administered respectively after the 137 Cs -- gamma ray whole body irradiation. By detecting the body weight, organ index, organ SOD activity, MDA content, peripheral blood （WBC, RBC, Hb, PLT）, the effect of KGF and G - CSF on irradiated mice blood and major organs were analyzed. Results After irradiation, the weight of KGF group and G - CSF group picked up more clearly;Compared with G - CSF group, there was statistically significant difference in lung index combined group （P 〈 0.05） ; Content index of peripheral blood RBC, Hb, PLT, combined group was higher than that of the other group ; liver, heart SOD activity in G - CSF group is higher than that of KGF group and combined group, lung SOD activity in KGF group is higher than that of G - CSF group and combined group. Conclusion This study shows that of the mice hematopoietic system restoration in the combined is better than that of the other groups ： G - CSF is more effec- tive for the liver and heart antioxidant capacity; KGF is more effective for pulmonary antioxidant capacity.

关 键 词：角质细胞生长因子(KGF) 粒细胞集落刺激因子(G-CSF) 放射损伤 放射防护作用 

分 类 号：Q691.9[生物学—生物物理学]