吲哚丙酮酸的酮-烯醇互变异构化的NMR研究  

An NMR Study on Keto-Enol Tautomerism of Indole-3-Pyruvic Acid

作　　者：杨晓艳[1,2] 吴香玉[1,2] 安艳捧[1,2] 唐惠儒[1] 

机构地区：[1]中国科学院生物磁共振分析重点实验室,波谱与原子分子物理国家重点实验室,武汉磁共振中心(中国科学院武汉物理与数学研究所),湖北武汉430071 [2]中国科学院大学,北京100049

出　　处：《波谱学杂志》2014年第1期81-90,共10页Chinese Journal of Magnetic Resonance

基　　金：国家自然科学基金资助项目(21175149;21221064);国家重点基础研究发展计划资助项目(2010CB912501)

摘　　要：吲哚丙酮酸是色氨酸代谢途径中的一种重要α-酮酸,但其酮-烯醇互变异构化现象及互变异构体的NMR数据尚未见报道.该文采用多维NMR技术研究了吲哚丙酮酸在不同溶剂中主要存在形式.使用2D NMR谱对吲哚丙酮酸的烯醇式和酮式结构进行了NMR研究.结果表明吲哚丙酮酸在乙腈溶液中主要以烯醇式结构存在,而在水溶液中主要以酮式结构存在.Indole-3-pyruvic acid is an important a-keto acid in the tryptophan pathway. In this work, two main forms of indole-3-pyruvic acid in acetonitrile and water solutions were studied using multi-dimensional NMR techniques. The results showed that both forms were present, and the enol-form of indole-3-pyruvic acid was the dominant form in acetontrile whereas the keto-form was the main form in water. This work provided detailed assignments for the 1H and 13C NMR signals from both the enol- and keto-forms of indole-3-pyruvic acid, offering essential spectroscopic information for this metabolite.

关 键 词：液体磁共振(NMR) 归属 2D NMR 吲哚丙酮酸 

分 类 号：O482.53[理学—固体物理]