大鼠血浆中甘草次酸的HPLC法测定及其药动学  

Determination of Glycyrrhetinic Acid in Rat Plasma by HPLC and Its Pharmacokinetics

作　　者：郝春影[1,2] 郭一飞[1] 赵燕娜[1] 李艳红[1,2] 王向涛[1] 

机构地区：[1]北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室,北京100193 [2]黑龙江中医药大学药学院,黑龙江哈尔滨150040

出　　处：《中国医药工业杂志》2014年第3期258-260,共3页Chinese Journal of Pharmaceuticals

摘　　要：建立了高效液相色谱法测定大鼠血浆中的甘草次酸,并考察其在大鼠体内的药动学.采用Symmetry（R） Shield C18色谱柱,以乙腈：0.1％磷酸为流动相,梯度洗脱,检测波长254 nm.甘草次酸在0.05～10 μg/ml浓度范围内线性关系良好;高、中、低浓度溶液的日内和日间RSD均小于3.4％;提取回收率为89.5％～107.9％,RSD为2.2％～4.6％.大鼠灌胃给予甘草次酸（50 mg/kg）后,血浆中甘草次酸的药动学参数分别为：cmx （28.48±6.90）μg/ml,tmax（3.00±1.07）h,t1/2（9.23±4.91）h,AUC0→∞（306.57±68.42） h·μg·ml-1,AUC0→t （290.93±68.36） h·μtg·ml-1.An HPLC method was established for the determination of glycyrrhetinic acid in rat plasma and its pharmacokinetics in rats was investigated. A Symmetry Shield C18 column was used with the mobile phase of acetonitrile ： 0.1% phosphoric acid by gradient elution at the detection wavelength of 254 nm. The calibration curve was linear in the concentration range of 0.05 - 10 μg/ml, with the intra- and inter-day RSDs less than 3.4%, and extraction recoveries of 89.5% - 107.9% （RSDs of 2.2% - 4.6%）. After oral administration of 50 mg/kg to rats, the main pharmacokinetic parameters were as follows： cmx （28.48±6.90）μg/ml,tmax（3.00±1.07）h,t1/2（9.23±4.91）h,AUC0→∞（306.57±68.42） h·μg·ml-1,AUC0→t （290.93±68.36） h·μtg·ml-1.

关 键 词：甘草次酸 高效液相色谱 药动学 测定 

分 类 号：O657.72[理学—分析化学] R969[理学—化学]