一个水稻矮秆窄叶突变体的鉴定和基因定位  

Analysis and mapping of a dwarf and narrow leaf rice mutant

作　　者：刘继云[1,2] 叶胜海[2] 李小华[2,3] 赵小燕[1,2] 尚海漩[1,2] 纪现军[2,3] 邓晓梅[2,3] 陈萍萍[2] 金庆生[2] 张小明[2] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021 [3]杭州师范大学生命与环境科学学院,浙江杭州310036

出　　处：《浙江农业学报》2014年第1期7-13,共7页Acta Agriculturae Zhejiangensis

基　　金：浙江省科技厅项目(2012C12901-2);国家自然科学基金项目(31071207)

摘　　要：常规粳稻秀水09经过甲基磺酸乙酯(EMS)诱变处理,获得了一个矮秆窄叶突变体dnl1(dwarf and narrow leaf 1),经多代自交性状稳定。dnl1突变体与野生型相比,具有植株矮、叶片窄、分蘖强的特点。遗传分析表明dnl1突变性状受1对隐性核基因控制。通过图位克隆的方法,利用SSR分子标记将突变基因dnl1定位在第4号染色体SSR标记RM17478与RM17488之间约260 kb范围内。测序比对分析表明Dnl1可能为Nal1基因的等位基因。A dwarf and narrow leaf （dnl1） mutant was isolated by ethylmethylsulfonate （EMS） treating for a japoni-ca rice variety Xiushui 09 dry seeds,it could be inherited stably after several selfing .Compared with its wild type Xi-ushui 09, the mutant shows dwarf , narrow leaf and more tillers .Genetic analysis showed that the traits of the dnl1 mutants were controlled by one pair of recessive nuclear gene .With map-based cloning approach , Dnl1 gene was mapped between SSR marker RM 17478 and RM17488 on the chromosome 4, a 260 kb region.Sequencing and blast analysis indicated that Dnl1 is an allele to the gene Nal1.

关 键 词：水稻 矮秆窄叶 图位克隆 分子定位 

分 类 号：S511[农业科学—作物学]