瓦松快繁体系的构建研究  

Construction of rapid propagation system for Orostachys fimbriatus

作　　者：李庆伟[1] 侯殿明[1] 梁明勤[1] 刘荣宁[1] 

机构地区：[1]河南农业职业学院现代农业工程系,河南郑州451450

出　　处：《浙江农业学报》2014年第1期84-88,共5页Acta Agriculturae Zhejiangensis

基　　金：河南农业职业学院2011年重点项目(HNAC2011KY002)

摘　　要：以瓦松为研究材料,系统地研究了丛生芽的诱导、增殖及生根培养等,探索瓦松快繁的最佳途径并构建其快繁体系,结果表明:茎段接种于MS+1.0 mg·L-16-BA+0.2 mg·L-1IBA+20 g·L-1蔗糖培养基上,茎段增殖倍数可达11.69,叶片接种于MS+1.5 mg·L-16-BA+0.1 mg·L-1IBA+30 g·L-1蔗糖培养基上,能产生很好的不定芽,其再生芽数达9.71。在1/2 MS+0.2 mg·L-1IBA+0.05 mg·L-1NAA培养基中,生根率达100%,平均每株能产生12.3条根,植株生长健壮。Studies on the multiple shoots induction , propagation and rooting of Orostachys fimbriatus plantlet in vitro were conducted to construct a rapid propagation system for Orostachys fimbriatus.The results were as follows： the proliferation multiple of the stem was 11.69 when cultured with the MS＋1.0mg· L^-1 6-BA＋0.2mg· L^-1 IBA＋20g · L^-1 sucrose medium.Leaves inoculated on MS ＋1.5mg· L^-1 6-BA＋0.1mg· L^-1 IBA＋30g· L^-1sucrose medium could regenerate small adventitious buds well , and the number of regenerated bud per leaf was 9.71 .The vigorous proliferous shoots were inoculated on 1/2 MS＋0.2mg· L^-1 IBA＋0.05mg· L^-1 NAA medium for rooting, and the rooting rate was 100%, the root number was 12.3 per shoot .

关 键 词：瓦松 快繁 丛生芽 生根 

分 类 号：Q949.751.1[生物学—植物学]