Regulatory Effect of Electroacupuncture on Ceramide Galactosyltransferase Expression in Sciatic Nerve of Experimental Diabetic Rats  

作　　者：Jiang Chao Liu Ping Zhang Yun-yun Zhang Qiu-juan Huang Gao-min 

机构地区：[1]Department of Geriatrics,Shaanxi Provincial Hospital of Traditional Chinese Medicine [2]Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine [3]Yueyang Hospital of Integrated Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine

出　　处：《Journal of Acupuncture and Tuina Science》2014年第1期1-7,共7页针灸推拿医学（英文版）

基　　金：supported by Postgraduates Innovation Ability Training Special Scientific Research Project of Shanghai University of Traditional Chinese Medicine

摘　　要：Objective: To observe the change of ceramide galactosyltransferase(CGT) expression in sciatic nerve of experimental diabetic rats after electroacupuncture(EA) treatment. Methods: The rat model of diabetic peripheral neuropathy(DPN) was established with Streptozotocin(STZ). Twenty-eight rats were randomly divided into a normal group, a model group, an EA acupoint group, and an EA non-acupoint group, with 7 rats in each group. Acupuncture on points Shenshu(BL 23) and Zusanli(ST 36) was performed in the EA group using EA electronic device with continuous wave, 2 Hz, 20 min every time, once every other day for 12 times. Acupuncture(on the tip of rat tail) in EA non-acupoint group was performed with the same electronic parameters and time. The model group and the normal group were not given any treatment, except the same fixation as that in the EA acupoint group. After 12 treatments, the albumen and mRNA expressions of CGT of sciatic nerve in rats from each group were measured with real-time polymerase chain reaction(RT-PCR), fluorescence quantitative PCR(FQ-PCR) and immunohistochemical methods. Results: After modeling, the CGT expression was significantly lower than that in the normal group(P<0.01); however, after 12 EA treatments, the CGT positive cells in the EA acupoint group were up-regulated and significantly higher than those in the EA non-acupoint group and the model group(P<0.01). The CGT mRNA expression of the rats after modeling was also higher than that of the normal rats(P<0.01), and the expression in the EA acupoint group was significantly lower than those in the model group and the EA non-acupoint group(P<0.01, P<0.05). Conclusion: EA treatment with acupoints displays some therapeutic or preventive effect in the recovery of DPN via reversing the abnormal expression of CGT mRNA in sciatic nerves of experimental diabetic neuropathy rats, and it is worthy of further study.Objective： To observe the change of ceramide galactosyltransferase（CGT） expression in sciatic nerve of experimental diabetic rats after electroacupuncture（EA） treatment. Methods： The rat model of diabetic peripheral neuropathy（DPN） was established with Streptozotocin（STZ）. Twenty-eight rats were randomly divided into a normal group, a model group, an EA acupoint group, and an EA non-acupoint group, with 7 rats in each group. Acupuncture on points Shenshu（BL 23） and Zusanli（ST 36） was performed in the EA group using EA electronic device with continuous wave, 2 Hz, 20 min every time, once every other day for 12 times. Acupuncture（on the tip of rat tail） in EA non-acupoint group was performed with the same electronic parameters and time. The model group and the normal group were not given any treatment, except the same fixation as that in the EA acupoint group. After 12 treatments, the albumen and mRNA expressions of CGT of sciatic nerve in rats from each group were measured with real-time polymerase chain reaction（RT-PCR）, fluorescence quantitative PCR（FQ-PCR） and immunohistochemical methods. Results： After modeling, the CGT expression was significantly lower than that in the normal group（P〈0.01）; however, after 12 EA treatments, the CGT positive cells in the EA acupoint group were up-regulated and significantly higher than those in the EA non-acupoint group and the model group（P〈0.01）. The CGT mRNA expression of the rats after modeling was also higher than that of the normal rats（P〈0.01）, and the expression in the EA acupoint group was significantly lower than those in the model group and the EA non-acupoint group（P〈0.01, P〈0.05）. Conclusion： EA treatment with acupoints displays some therapeutic or preventive effect in the recovery of DPN via reversing the abnormal expression of CGT mRNA in sciatic nerves of experimental diabetic neuropathy rats, and it is worthy of further study.

关 键 词：Acupuncture Therapy Electroacupuncture Sciatic Nerve Diabetic Neuropathies N-Acylsphingosine Galactosyltransferase 

分 类 号：R2-03[医药卫生—中医学]